What concentration of agarose can be used for the mouse skin agarose embeding for vibrotome sectioning?

Ravindra Kailasrao Zirmire @Ravindra-Zirmire

13 May 2015 1 6K Report

I am trying to do the vebrotome sectioning of the mouse skin.But at 4% agarose concentration sectioning is not working well.

Wolfgang H. Muss

Ravindra, it would be helpful to see some other details of your technique/methods used (size of your skin samples, guessing you would like to get vibratome sections in the orthogonal plane, Vibratome machine you use, why embedding in Agarose?, etc.)

Regards, Wolfgang

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