I am working with lymphatic endothelial cells and trying to test effect of few chemicals, on LECs by wound healing scratch assay. My confusion is, if I have 4 different chemicals cited in different literature to be used for cell culture studies, in different concentration let's say, if A is 10 um then B 25, C may be 100 and D 400um. What would be ideal concentration if I want to compare the effect of these individual chemical on one cell type. Should I be choosing one from literature individually or a standard concentration for all to be used, to make a comparison.
Hi Gaurav,
I hope you are doing well.
Since the chemicals are different and performed by different research groups, I would recommend doing a pilot study with your cell culture conditions and a range of concentrations of A, B, C, and D. But definitely you can reduce the range of test concentration by referring the available literature.
I hope that helps.
Cheers
Nimal
Nimal Raveendran thanks buddy doing good m, how about you.
yes of course initial thought was to screen different dosage but later realized the range is very high but now thinking that’s the only way out.
Nimal Raveendran Thank you Dr Raveendran for helping me with your wisdom
- Badges
- Science topic
- Similar topics
- Biological Science
- Anatomy
- Tissue