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Questions related from Nimal Raveendran
I am using some primary human cells for my experiments, But the activity/proliferation rate of the cells in their late passages (>P8) are very low and I can not use them for long like cell...
17 December 2017 4,111 3 View
(a) What is the rationale of doing a slow freezing (controlled rate freezing)? (b) Why a quick thawing of frozen vials at 37 degree C? I read some research articles , but confused about...
17 December 2017 4,242 8 View
I want to study the effect of a hormone in stem cells. I know the dose (20 mg in 80 microL) used for I.V injection. How can I determined the dose that I have to use for cell culture studies? Can I...
03 February 2016 876 0 View
I want to increase lipophilic nature of protein nanoparticles . What are the chemicals that can be used to improve its lipohilic character? Which procedure I should follow?
08 November 2013 2,378 4 View
Adult stem cells, especially isolated from bone marrow, have been extensively investigated in recent years. Studies focus on their multiple plasticity of trans differentiating into various cell...
29 September 2013 3,765 1 View
I read about application of hydrogels in wound healing. But we can use different materials for hydrogel, out of these materials which one will shows bet healing?
29 September 2013 3,063 3 View
Generally we use gel doc for documenting gel images. But recently, I learned that some are using high pixel camera for taking gel images. How is this possible? Can we use high pixel camera as an...
12 September 2013 9,811 5 View
Using 'Highlight sequence features' option in NCBI, we have retrieved all CDS of a full genome. From this data set, CDS having less than 300 nucleotide were excluded. Now how should we proceed to...
05 September 2013 1,856 2 View
We want to isolate genomic DNA from plant leaves by using CTAB method. Unfortunately we don't have PVP. Can we use other chemicals instead of PVP to absorb polyphenols? How can we modify the...
05 September 2013 2,958 4 View
I am planning to cultivate algae in waste water for the omega oil production. I heard about different cultivation systems like open/closed ponds, photo bio-reactor, feremeter,etc. Eg: a, Algal...
27 August 2013 4,074 6 View
In Ayurveda some plants are very useful for the regeneration of damaged body parts. Whether these plants chemicals have any role in stem cell differentiation? Can we incorporate these...
20 August 2013 6,921 3 View
