Nimal Raveendran

6 Questions 8 Answers 0 Followers

Questions related from Nimal Raveendran

What defines the age of a primary cell line?

I am using some primary human cells for my experiments, But the activity/proliferation rate of the cells in their late passages (>P8) are very low and I can not use them for long like cell...

17 December 2017 4,090 3 View

Why Slow freezing and Fast thawing in Cryopreservation of mammalian cells?

(a) What is the rationale of doing a slow freezing (controlled rate freezing)? (b) Why a quick thawing of frozen vials at 37 degree C? I read some research articles , but confused about...

17 December 2017 4,217 8 View

How can I determine the dose for cell culture studies of a drug based on its I.V dose?

I want to study the effect of a hormone in stem cells. I know the dose (20 mg in 80 microL) used for I.V injection. How can I determined the dose that I have to use for cell culture studies? Can I...

03 February 2016 860 0 View

Which is the best encapsulation method of stem cells for liver regeneration ?

Adult stem cells, especially isolated from bone marrow, have been extensively investigated in recent years. Studies focus on their multiple plasticity of trans differentiating into various cell...

29 September 2013 3,758 1 View

How to find the total length of CDS?

Using 'Highlight sequence features' option in NCBI, we have retrieved all CDS of a full genome. From this data set, CDS having less than 300 nucleotide were excluded. Now how should we proceed to...

05 September 2013 1,843 2 View

Which chemical can replace the function of polyvinylpyrrolidone (PVP) in Plant Genomic DNA Extraction?

We want to isolate genomic DNA from plant leaves by using CTAB method. Unfortunately we don't have PVP. Can we use other chemicals instead of PVP to absorb polyphenols? How can we modify the...

05 September 2013 2,947 4 View