I grow cells on Silicon substrates as part of my research. However, i usually throw out the substrates afterwards. I was wondering if there is a way to clean them in order to re-use them, but without changing the micro and nano-scale topographies.
I tried alcohol or trypsin (which we use to remove cells) but it did not work (i can see that there is GFP signal after cleaning, and possibly lots of ECM proteins). I was wondering if there is a protocol you use.
Hi Sara,
I think that the piranha solution is an effective way to clean silicon substrates and remove organic materials from the surface of the substrate. The solution is prepared by mixing sulfuric acid and hydrogen peroxide with 3:1 (v/v). Please put sulfuric acid in a beaker first. Then, put the silicon substrate in the piranha solution for 10 minutes, and rinse it for 30 minutes with distilled water.
Best,
I agree with Alim Yolalmaz . Hence I would like to suggest you the following paper:
Article Waste Silica as a Valuable Component of Extensive Green-Roof...
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