Sara Mingu

4 Questions 7 Answers 0 Followers

Questions related from Sara Mingu

SH-SY5Y Cells growing too slowly. What can i do?

I seeded a flask of human neuroblastoma cells almost three weeks ago, and they did not grow to confluency yet. This is the second passage; the first time the cells took like 2 weeks to reach...

14 November 2019 3,958 2 View

What cleaning protocol will remove all biological material from Silicon samples?

I grow cells on Silicon substrates as part of my research. However, i usually throw out the substrates afterwards. I was wondering if there is a way to clean them in order to re-use them, but...

19 October 2019 5,669 3 View

How can i set up hybridization and ligation reactions to make a ~120 bp sequence from shorter, single stranded oligos?

We want to obtain a double stranded 120 bp long sequence and ligate it to a vector. The sequence is highly repetitive so we were not able to sequence it; therefore we were thinking of making it...

18 September 2019 4,461 3 View

How to obtain EGFP localization to the nucleus in N2A?

is there any nuclear localization signal which will retain EGFP in the nucleus of the N2A cells?

09 August 2018 3,561 2 View