Non motile black dots in animal cell culture, tightly adhered to the cells along the cell periphery. Is it a contamination? How to get rid of them?

I am seeing non-motile black dots of almost equal size along the periphery of skin melanoma cell line A375. They are present in almost all the cells. They don't turn the media turbid. However...

18 January 2021 5,151 1 View

Best flow cytometry markers for Thp1-derived M1 and M2 macrophages?

Hi everybody, I am trying to polarize and differentiate thp1 cells to M1 and M2 macrophages. There are lots of different protocols, so I've been trying different incubation times and...

02 November 2020 8,554 3 View

Have you ever seen two peaks after preparation small RNA library for NGS?

Hi, I have problem with my libraries, I use ClenTag Small RNA Library Preparation Kit by Trilink. The material is RNA from plasma cells. I have already done this two years ago and everythink...

25 August 2020 8,459 1 View

What are the key things to write and publish a paper in a good indexed journal?

The major spices and ingredients to add flavour in a paper for a journal in the domain of Computer Vision and Machine Learning.

27 June 2020 7,967 10 View

How to decide that a work is suitable for a conference or a journal in the field of Computer Science & Engineering?

What are the deciding factors to submit a paper in conference or journal.

21 June 2020 1,604 8 View

Whether Lambert Academic Publishing (LAP) provide printed copies to author? Do authors need paymet or sharing bank setails?

Hello Everyone !! Does anyone have any experience in publishing through Lambert Academic Publishing (LAP)? Recently I got a request mail from them regarding publication. I want to know about the...

25 April 2020 2,651 2 View

How can I write the .rpy and .jnl file in Abaqus cae for windows?

Hello :) I have the Abaqus CAE student version for Windows recently. My problem is: Abaqus does not write the .jnl and .rpy files automatically during a session as I am used to from the Linux...

30 March 2020 2,579 4 View

HSV1 in human tissue - dangerous after the fixation with PFA?

Hi, we are wondering about the HSV1 virus in human tissue. We want to perform in situ sequencing with this tissue, but we are not experienced with the virus-infected samples. Is it possible that...

14 October 2019 8,969 1 View

What details should be followed in MEGA-X for the Multiple Sequence Alignment of 16S data?

I have some 16S RNA sequence with which I want to do the phylogenetic analysis, so Initially I did BLAST against SILVA db and NACBI 16S db and went for MSA in MEGA-X using Muscle and Clustal both...

21 July 2019 9,320 3 View

What are the additional advantages that a Fano resonance possesses in comparison to normal dipolar resonance in Optical systems?

Fano resonance is a distinctive spectroscopic behavior in many optical systems. There're certain differences in terms of physics and behavior of Fano resonance compare to dipole resonance which is...

30 May 2019 4,244 2 View

ZnS oxidizes to ZnO!!why??

when I put my ZnS solution in the chemical bath, the ZnS oxidizes so there are ZnO peaks appearing because of the reaction with the oxygen in the air, despite the fact that I close the beaker...

14 February 2021 5,778 9 View

Charmm/GROMACS Residue 'ZN' not found in residue topology database?

I am trying to do some MD simulations of a protein with zinc ions. I am trying to use a charmm forcefield (I have tried 2017 and 2021) and Gromacs. I get an error when doing pdb2gmx Residue 'ZN'...

12 February 2021 9,367 2 View

Xrd result of ZnO thin film shown better crystallinity at low temperature and the crystallinity decreases as the temperature increased. why?

I have synthesized ZnO thin films at four different substrate temperature say, 300, 350, 400 and 450C. and the samples were annealed at 450C for half an hour. the xrd result shown better...

04 February 2021 7,641 4 View

Is it possible to prepare clay minerals by mixing pure oxides of required elements? What would be theoretical difficulties with it?

Practically this is not at all necessary. But theoretically, would hydrous/hydrothermal synthesis be enough (like mix 1 mole alumina gel and 4 mole silica gel and gently heat) or would it require...

26 January 2021 6,403 3 View

How can I prepare 10mM sodium acetate buffer with pH 9?

I have found an article (Extraction, physicochemical characteristics and functional properties of Mung bean protein) for extracting mung bean protein isolate using water as solvent. As my...

21 January 2021 8,995 3 View

I've my Gold Nanorods in solution form and I have to conjugate them with drugs. Please go through description i have given?

I've my Gold Nanorods in solution form and I have to conjugate them with drugs. They remain in solution form after conjugation. Now for MTT assay for drug delivery on cancer cell lines, I am...

16 January 2021 3,992 1 View

What is the basis set to optimize ZnO using GaussView for DFT calculation study?

Greetings, I have a bit of problem in setup the basis set of my DFT study for ZnO.. As you can see in the image attached below, I use B3LYP 6-31G (d,p) for geometrical opt. of ZnO.. But sadly, I...

13 January 2021 7,508 4 View

Can anyone suggest me solvent to dissolve EVA (Ethylene Vinyl Acetate) polymer?

I am working on experiment where i want EVA to dissolve in some solvent. can anyone suggest me solvent to use ?

06 January 2021 4,251 3 View

Please share some research papers on the synthesis of Mn3O4 nanoparticles via Hydrothermal method by using Manganese(II) nitrate hydrate?

05 January 2021 9,040 4 View

What is the advantage of nanostructured ZnO thin film over continuous ZnO thin film for optoelectronic application?

What is the advantage of nanostructured ZnO thin film over continuous ZnO thin film for optoelectronic application?

30 December 2020 9,422 2 View