Zone of inhibitions sometimes appear fuzzy/sharp with different antibiotic discs on the same agar plate, what are the explanations for this?
Also, how important are these when determining the best antibiotic for treatment?
Thanks
Our manuscript was published ahead of print. The edited and revised version is now available. How do I remove the old version of the manuscript from the research gate?
01 February 2021 5,965 1 View
I am analyzing data from a FISH experiment and notice that there are false negatives. What is the best option to change in the experimental set up to avoid this from happening?
04 October 2020 5,777 1 View
Hello, Maybe is a bit silly question but, I have a variable (N=78) and on shapiro-wilk test the sig. is .008 which is just over (greater) than 0.05 meaning that is just about normally...
29 August 2020 1,384 4 View
Do you first calculate the average of all the CFU and then sub it into the equation ? OR Do you calculate each individual log CFU/ml and then average that?
19 March 2019 7,956 6 View
How do you determine the upper detection limit for E. faecium? If there are too many to count on the plate, can I suggest that there are >25,000 CFU/ml for example? - or would that be too much
02 March 2019 1,261 1 View
I have a treated and a control condition of agar plates. I counted the number of colonies and wanted to carry out a mann whitney test. Do I carry out the mann whitney test using the counted...
02 March 2019 4,830 1 View
02 March 2019 1,412 7 View
For example, do isopropanol/1-propanol/ethanol alcohol based hand rubs all use the same mechanisms to kill bacteria? Do they all just disorganize lipid membranes, denature proteins, and...
02 March 2019 8,814 3 View
I'm trying to use homology modelling to find the structure of the N-terminus of a protein which is thought to contribute to its hetero-oligomeric structure. However, I can't find an x-ray...
24 December 2018 6,810 3 View
Why is that a higher affinity leads to increased effectiveness of a drug inhibitor? Also, most importantly why do enzymes bind more tightly to transitions state analog inhibitors? what is...
11 December 2016 2,057 3 View
Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...
01 March 2021 2,027 2 View
Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...
28 February 2021 7,292 3 View
The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....
27 February 2021 4,450 2 View
I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...
25 February 2021 3,221 7 View
Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...
24 February 2021 9,839 3 View
Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...
24 February 2021 3,157 6 View
Hi everyone, I need to expression of GFP with pET28 plasmid in E. coli BL21(DE3) plysE. Transformed cells have kanamycin and chloramphenicol resistance. Normally, only transformed cells should...
22 February 2021 4,032 4 View
I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...
16 February 2021 8,519 5 View
I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...
16 February 2021 169 4 View
I am doing a salt tolerance-related experiment and for that I need to relate my salt levels to that in bacto agar. I don't want my salt concentrations to increase due to already salt content...
09 February 2021 4,158 1 View