What can one do in origin if the curve fitting is good but the error in the peak parameters is more than the actual parameter calculated?

02 August 2019 0 6K Report

I am trying to fit my XRD data using multiple peak fit in origin software by pseudo voigt function. Cumulative peak is following the curve but the dependency in parameter is too high (almost 1) and error in calculation is very high.

Badges
Science topic

More Abhishek Thakur's questions See All

"A Markov-like Model for Patient Progression"?

A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...

05 August 2024 10,079 0 View

How to increase protein lysate concentration?

I am trying to prepare samples for IP. The pellet size is good and I am using 1% Tx-100 and using a syringe to further lyse the cells and maintain protein-protein interactions. I keep having a...

01 August 2024 7,199 1 View

La animación digital en plataformas digitales?

Hoy la animación se utiliza como una tecnología multimedia con gran potencial educativo, que va mucho más allá de sólo crear figuras, ya que puede promover una mejor comprensión en...

01 August 2024 7,186 0 View

GSH estimation assay: What is the right choice of standard?

Hi there, My question is: What standard curves should be used while estimating Tot GSH and GSSG by kinetic method using GR enzyme mediated recyling with DTNB chromophore? Actually I am following...

01 August 2024 8,217 1 View

How to do pca analysis of c-alpha atom of the protein?

i m interested in pca analysis of c-alpha atoms in gromacs for that i used the following gmx_mpi covar -s mdca.tpr -f mdca.xtc -o eigenvalca.xvg -v eigenvecca.trr -av average.pdb -n index.ndx but...

30 July 2024 1,607 1 View

What exactly is RAG-LLM doing? Isn’t it data engineering?

What exactly is Retrieval Augmented Generation for Large Language Model doing? Isn’t it data engineering?

30 July 2024 7,376 3 View

After a lot of feature engineering for CTR modeling, it feels like it's basically the end of iteration? I mean, it's not cost-effective to keep doing?

After a lot of feature engineering for click-through rate modeling, it feels like it's basically the end of iteration? I mean, it's not cost-effective to keep doing it?

29 July 2024 4,955 0 View

Is space-time dilation conceptually equivalent to space-time expansion?

Relativistic space-time is described as a four-dimensional continuum comprising three dimensions of space and one dimension of time. In this framework, space and time are interwoven, forming an...

29 July 2024 5,365 2 View

I have synthesized a composite of ZnO and In2O3 with variations in ZnO content. In Raman , what is the peak observed at 130 cm-1?

Dear all, I have noticed, as the ZnO content increases, the Raman intensity at 100cm-1 also increases. However, there is a peak nearby at 130 cm-1, which I could not find in any paper related to...

28 July 2024 5,410 1 View

How to estimate sample size for GWAS of continuous and discrete traits? What are the pre-requisites?

Genome-wide association study (GWAS) Continuous traits: eg. Height Discrete traits: eg. Eye color

28 July 2024 286 0 View

Standard curve of H2O2?

Hi, I need to prepare a standard curve of H2O2 to evaluate the primary products of oxidation. H2O2 I have is 35%. How do I prepare a stock of H2O2 10 microlitere. If s.o has experience, I would be...

26 July 2024 3,460 2 View

Can I use soybean, sunflower, or olive oil to prepare a standard curve for lipid estimation in plants or pollen?

I am trying to analyze lipids in plants; specifically pollen by using Sulfo-Phospho-Vanillin (SPV) Method! Can I prepare a standard curve for this estimation using soybean, sunflower, or olive...

01 July 2024 3,267 1 View

Generating multiple standard curves from a single template?

Hi everyone! After thinking about it on my own, I'm posting a question here. Has anyone ever tried creating qPCR standard curve samples (plasmid or DNA standard) by inserting multiple target...

30 June 2024 1,569 1 View

About the standard of the FRAP in calculation?

Good morning/day/evening salaam I’d like to ask about the FRAP assay again. I have read some articles which represent the data of frap assay as mg trolox equivalent/L, mg ascorbic acid...

27 June 2024 5,239 0 View

can I use Roc Curve in a pilot study?

Hi everyone, we know that the analysis of pilot studies should be based on descriptive statistics and ideally not involve any inferential statistics (i.e., P values). Based on this can I use Roc...

24 June 2024 4,402 7 View

How to increase qPCR efficiency to acceptable range?

My aim is to create a standard for qPCR for absolute quantification of DNA extracted from soil. The reaction contains PowerUp SYBR Green Master Mix, BSA, primer pair (A189F-MB661R- 0.67 uM), and...

10 June 2024 6,708 9 View

XPSpeak41 after optimizing the graph is still not well fit?

Hi, i am currentyl fitting mno2 material into the peak at Mn 2p 3/2 and Mn 2p 1/2. in the right side of the peak, the tip highlighted in red is still not fit enough. is there any idea how to fit...

05 June 2024 7,957 6 View

What is Area Under the Curve for detecting SARS-COV-2 neutralizing antibodies?

I have a question about AUC for calculating SARS-CoV-2 neutralizing antibody tests. AUC here is not the Area Under the ROC Curve. After six serial dilutions of human serum, an indirect ELISA was...

05 June 2024 8,191 0 View

How can I determine the cut off from the ROC curve?

Hello, If I have the ROC curve from SPSS output, How can I determine the cut off?

01 June 2024 9,670 3 View

Complex dynamic systems theory data analysis inquiry?

How many participants and times are required to conduct latent growth-curve modeling or cross-lagged panel analysis. Thanks in advance.

29 May 2024 1,853 1 View