I've performed midi preparation and DNA purified using invitrogen PureLink™ HiPure Plasmid Midiprep Kit, but when testing 260/280, one sample yield value of 1.6 and another with 3.57.

I currently have no resource to perform 260/230, therefore I can only guess that 1.6 may due to salt contamination and 3.57 for RNA.

I'm now thinking about adding extra RNase(10ul) for sample(160ul---50ug/ul) of 3.57 and repeat ethanol step twice(20000g 30min 4'C), but I have no clue for sample of 1.6(160ul---2ug/ul).

Can someone give me an advice?

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