is there a particular chemical that can be used to block the oxidation of leucomethylene blue to methylene blue at pH 7.4?
I have been trying to check the overexpression of the antitoxin of my chosen TA system of mycobacterium sp. I have tried to induce the clone at 20, 25 and 37° C at 0.1, 1 and 10 mM concentration...
22 May 2024 1,334 0 View
I'm using Ni-NTA resin (Qiagen) column to purify my his-tagged protein. Over a period of time the color of my column has changed to color less. I'm using the concentration you can see in the...
15 May 2024 2,573 4 View
I am trying to recharge the Ni-NTA column for his-tagged protein purification but my NiSO4(H2O)6 is not dissolving in 1x PBS and I have tried it from a different reagent bottle also, please help!
15 May 2024 9,746 3 View
Tourism industry is a big service provider and lot of scope for generating different types of employment
15 August 2023 8,271 2 View
Hi everyone, I'm a newbie here and to writing researches as well. I'm currently engaged in writing my first research (dissertation) for academic purposes and need some help with the same. Here...
29 April 2023 844 1 View
Hi We have developed a set of 2 plant extract based oils. Topical application of the oils have shown efficacy against RNA enveloped family of viruses as well as on 1100+ covid symptomatic...
21 July 2020 1,652 0 View
I need to add NiCl2 to PBS for an assay but it causes turbidity, possible due to precipitates of nickel phosphate. is there a way to prevent this?
20 July 2016 7,431 1 View
I am testing MCF -7 cells for lipoxygenasev activity but cant find any literature that reports the specific activity that these cell have. Any input will be appreciated. Thanks
29 June 2016 842 2 View
I am running an experiment to detect ammonia produced in a biological system die to the action of an enzyme using the Berthelot reaction. It seems like the addition of detergent NP-40 to my...
21 June 2016 9,045 2 View
I am growing MCF-7 cells and after 4 hours of thawing and putting in a flask, the cells are still floating. I am worried because this is an adherent cell line and should not take this long to...
17 June 2016 4,308 3 View
Hello, I suspect that a molecule I am using is causing bacteria to experience osmotic shock. What chemical properties should I look for to compare this capability with those of other substances?...
06 August 2024 977 1 View
I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...
05 August 2024 3,290 1 View
Hi RG family. My team and I are working on some SCOPUS publications and we need co-authors who are willing and capable of undertaking both qualitative and quantitative-based studies. The scope...
02 August 2024 7,843 0 View
Hi RG family. My team and I are working on some SCOPUS publications and we need co-authors who are willing and capable of undertaking both qualitative and quantitative-based studies. The scope of...
02 August 2024 8,572 0 View
We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...
30 July 2024 942 2 View
Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.
29 July 2024 4,374 1 View
I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.
29 July 2024 8,560 0 View
My photocatalyst from solid waste red med. Dye is methylene blue My all parameter study is showing zero order. How to prove it further that the reaction in zero order?
29 July 2024 7,404 1 View
The column is used in Ion Exchange Chromatography of Monoclonal antibodies and the method of analysis is based on pH gradint.
13 July 2024 7,462 1 View
Hello! Have you ever observed that when measuring the viability of freshly thawed PBMC with trypan blue, the value is higher than with Annexin/PI staining or some other staining that takes into...
10 July 2024 640 1 View