I need to prepare bold basal medium for the cultivation of a microalgae Chlorella species, but want to know if I can use something like e.g cobalt chloride as a cobalt nitrate substitute.
Dear Dewald De Villiers many thanks for your very interesting technical question. Yes, cobalt(II) nitrate can be replaced by cobalt(II) chloride in Bold’s Basal Medium. This has been reported in the following research article:
Enhanced Vitamin B12 Production using Chlorella vulgaris
Article Enhanced Vitamin B12 Production using Chlorella vulgaris
This paper has been posted on RG as public full text and can bee freely downloaded as pdf file.
God luck with your research! 👍
Cobalt chloride is a common added source of Cobalt in many algal growth media. Shouldn’t present any issue, and typically added at low concentration, so substitutiin of chloride for nitrate won’t make a significant difference to anion balance.
Dear Chris Bolch
Algae need only water and sunlight for growth, it does not matter how many nutrients available in the water. but at the same time, the available nutrients support the variation (high or low) in algal growth rate. BBM is defined based on their research, you may try with different components and formulate a new culture media. you can do an experiment by substituting the Cobalt chloride or some other components likewise.
Good luck for your research...
I alway subsititute it for CoCl2. Its contribution to total nitrate is negligible.
Yes sir. You can use CoCl2. I am using BBM for culturing Scenedesmus. As it was adding in a low concentration, no significant difference in the growth rate and biomass observed.@Chris Bolch
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