Dewald De Villiers

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Questions related from Dewald De Villiers

Why would Chlorella sorokiniana have white pigment/lose pigment under mixotrophy?

I've been growing this species in Bold Basal Media, as many papers recommend, with supplemented glucose (10 g/L) under constant 40W light. For some reason the microalgae is not growing green. I've...

22 June 2021 7,264 0 View

What can be used as a substitute for Cobalt nitrate in bold basal media for Chlorella cultivation?

I need to prepare bold basal medium for the cultivation of a microalgae Chlorella species, but want to know if I can use something like e.g cobalt chloride as a cobalt nitrate substitute.

14 May 2021 8,365 5 View

What is an efficient way of surface sterilizing plant roots for later endophyte extraction?

I've tried various methods of using bleach, ethanol and some tween added too. I'd like to know a method which is able to yield effective results without washing out the endophytes.

16 February 2020 7,725 6 View