one reason can be the involvement of other gene of mismatch repair system. is there any other reason ?
Maybe do a PCR amplification of the protein genes . While the antibody used for IHC may detect a mutated protein, the protein itself may not be functioning properly.
we have done pcr but still in many samples we didnot get any mutation in either of the 4 mmr gene.
I have data set consist of x and y variable where I wan to perform maximum likelihood estimation (MLE) to fit a mean function and astandard deviation function o the data. I am estimating the the...
21 April 2024 1,734 7 View
Is it possible to cure diabetes clinically? There are huge number of diabetes patients around the world. what are the main causes of this medical conditionand what about history of diabetes. when...
01 March 2024 6,084 7 View
In a meta-analysis, there is just one study that reported the incident rate ratio, can I consider it as HR?
03 November 2023 1,845 4 View
I have a problem with it, as the whole solution changes colour and the reduction occurs in the whole solution. I just want that the particles get deposit on the Ti naotubes grown on Sheet.
20 October 2023 9,576 2 View
The dataset I am working with consists of two columns, X and Y where the function Y changes for increasing values of the X variable. If Y shows variations with X, the simplest way to do this is...
21 August 2023 1,088 10 View
I conducted a 100ns simulation of my docked complex, obtaining a maximum RMSD of 4.2 Angstroms and an average of 3.1 Angstroms. Can someone help me how can I justify this high RMSD? I have...
30 July 2023 4,291 13 View
hello! I am Momina Naz, from Capital University Of Science and technology. I need permission for Academic Stress Scale by kim 1970 For my research purpose but unfortunately i did’nt find author’s...
25 July 2023 9,335 0 View
Anyone who can help me in transfection of A549 cells. I have done several times but it doesn't work properly. Thank you so much.
16 July 2023 10,010 5 View
Medium Composition: Could you kindly suggest a medium formulation that has proven effective in inducing phenazine production? Please include the specific carbon and nitrogen sources, inorganic...
25 June 2023 3,191 1 View
Hi everyone Can anyone will describe the best protocol to label the membrane with N-(lissamine rhodamine B sulfonyl)-phosphatidylethanolamine (N-Rh-PE). I have to do in cardiac cells(primary and...
22 June 2023 790 0 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View