What can be the reason for the absence of the internal control band in the negative control in PCR?

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Sampada Sudarshan

If you have probably included the IC during extraction of nucliec acid for all samples and have forgotten to include your NC during extraction, it could be the reason for absence of IC in your NC !! Since you might have directly taken water or Negative template control for PCR directly rather than including that for nucleic acid extraction step (which includes addition of IC). Could you be more clear in what protocol you followed i.e.when you use the IC ? That way we could help you better.

Waqas Ahmed

Please specify your protocol, why do you want to have IC band in NC?