Sampada Sudarshan

3 Questions 20 Answers 0 Followers

Questions related from Sampada Sudarshan

Which is the best IHC Secondary detection system kit?

I'm performing IHC on mouse tissue. My primary antibodies are rat anti-mouse antibody. I would like to know which is the best secondary anti-rat HRP conjugated DAB detection system ki. There are...

11 November 2013 6,075 6 View

Which is the best company for ordering antibodies to immunophenotype mouse tissue by IHC ?

I am planning to immunophenotype mouse tissue that is infected with virus by Immunohistochemistry. I would like to know which company's antibodies works best for murine tissues? I have found a...

10 October 2013 1,888 24 View

Should acetone remain at room temperature while adding to the mammalian cell monolayer before incubation at 4 degrees for fixation?

I am working with BHK-21 cell line infected with virus and have to perform immunofluorescence to identify the same. I am using acetone for cell monolayer fixation by incubating the plate at 4...

06 June 2012 7,384 18 View