What can be the appropriate annealing temp for PCR if tm of forward primer is 57.9 and that of reverse primer is 60?

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Could be estimate or simulated the electrical properties of thin films obtained from new organo-metallic compounds?

In the context of synthesized organo-metallic compounds, I am interested in the formation of thin-films over silicon wafers. Among another characteristics to be investigated, electric properties...

13 January 2021 7,575 2 View

ANSYS, how is far-field acoustic pressure calculated (from which underlying surface)?

In ANSYS, as in also e.g. COMSOL Multiphysics, you can calculate a far-field acoustic pressure outside the explicit FEM domain. However, whereas in COMSOL Multiphysics you define a certain closed...

04 January 2021 3,076 8 View

How to interpret the quality level of animal studies by using CAMARADES tool?

We have been working in a systematic review of animal studies and are performing risk of bias and quality assessment using SYRCLE and CAMARADES tools, respectively. Thereby, we would like to...

26 October 2020 7,171 1 View

How to sample maize leaves and transporting them for specific leaf area measurements?

Dear RG community, I would like to sample (cut-off) maize leaves in the field and transport them to the lab for SLA (specific leaf area) measurements. After a brief literature review, I could not...

18 August 2020 6,079 4 View

Is it possible to predict in vitro / in vivo correlations for extended-release tablets by using PK data from the literature?

We have been dealing with the investigation of the effects of splitting extended-release tablets in the in vitro dissolution profile of drugs. Thence we are wondering whether the differences...

29 February 2020 2,530 2 View

How to find and extract a homogenous (uniform) area of pixel values in an image?

Dear RG Community, I am currently working with thermal images of natural scenes. Throughout the scene I positioned large artificial targets that are painted black, grey and white. I would like...

12 November 2019 3,772 13 View

Is there a way to obtain apart from Energy values and bond lenght values bond angle values from an IRC on Gaussian in order to plot a 3D PES?

I have been performing several IRC calculation for multiple TS on g16 and I obtain bond lenght values and Energy values in order to plot a 2D PES. Can I also obtain bond angle values from that...

09 June 2018 9,892 3 View

Always OOB sampling in R caret package when using random forests?

Dear RG-community, I am curious how exactly the training process for a random forest model works when using the caret package in R. For the training process (trainControl()) we got the option to...

28 May 2018 6,622 4 View

How to use a geographical (GIS) time-dependent dataset as boundary condition in Comsol?

I want to apply a time-depended geographical dataset (format: "Lon,Lat,Time,Data") as boundary condition in a Comsol model. How can a achieve this for an instationary simulation? I know that...

21 May 2018 7,783 3 View

Hi, is there anyone who wants assistance with interpretation and/or classification of mutations to determine possible causality?

While doing research on genes possibly involved in a specific phenotype, you can run in to several difficulties for the interpretation and classification of mutations found, consequently making...

12 October 2017 8,599 2 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...

25 February 2021 5,712 3 View