What can be the appropriate annealing temp for PCR if tm of forward primer is 57.9 and that of reverse primer is 60?

More Rene Christena's questions See All

Could be estimate or simulated the electrical properties of thin films obtained from new organo-metallic compounds?

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Is it possible to predict in vitro / in vivo correlations for extended-release tablets by using PK data from the literature?

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How to find and extract a homogenous (uniform) area of pixel values in an image?

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Hi, is there anyone who wants assistance with interpretation and/or classification of mutations to determine possible causality?

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How do I increase the yield of my PCR product?

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Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

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How to address this issue in the analysis of Real-Time PCR results?

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No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

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28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

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Calculating volume of DNA required from DNA concentration?

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Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

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Nadine A. Gund

Hi, the annealing temperature should be 54-56 °C (you can do a gradient PCR). Cheers, Nadine

Sibtain Afzal

Hi Rene

You may do the touchdown pcr. You will get your required results definetely.

Best Regards

Ananda Ayyappan Jaguva Vasudevan

I would average it so it is 59 degrees, and Annelaing tem=Tm-5deg; so I would try from 54degrees.

Md Suhail Alam

I would go for gradient PCR from 52-58 with a increment of 1degree.