I am working on determining cell viability following a resazurin-based toxicity assay. Some of the wells on the 96 well plate contained media, but no cells which I am using as a control. I have averaged out the fluorescence values for these wells and have subtracted this value from all of the other values to account for background signal. However, in doing so, I have some wells with negative values. I am able to calculate % cell viability per treatment, but the statistics are thrown of as a result of these negative values. How do I account for this?
Hi i have had same issues though not with resazurin. i measure fluorescence with porphyrin dyes. It all depends on the number of replicate wells if you have at least 4 replicate wells simply mask the one with the negative value and calculate the average for the remaining
three. thanks
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