I am standardizing an assay for thiopurine metabolite (TGTP, TGDP and TGMP) analysis from RBCs on HPLC with fluorescence detection. Can anyone suggest a suitable internal standard which can be used with fluorescence detection.
Please look at the following below links which may help you in your analysis:
https://www.ncbi.nlm.nih.gov/pubmed/18295998
https://www.shimadzu.eu/sites/shimadzu.seg/files/shimadzu_application_handbook_clinical_web.pdf
Thanks
Thank you Isam Eldin Hussein Elgailani . I will go through the shared references.
I think that ,if possible to work on Fe(III) ion in Heam or in red cells and Luminol reagent can detect it by fluorescence technique
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