I start the analysis of gene expression and I do not know how to plan the experiment. I know about the dilutions, color compensation but I do not know what is the calibrator and what material I should use for that?
Thank you, yest it helps.
Hello, I noticed a problem with my HPLC analysis and wanted to ask for help. During my analysis I've noticed repetitive problems with the baseline: it is dropping and then (usually) going back...
20 May 2024 6,501 3 View
Hello I am looking for information about osmophilie microorganisms and specifically their enzymes. If there are any industrially important enzymes from microorganisms living in environment where...
19 May 2024 136 1 View
I am preparing an experiment in which I would like to sample roots of sunflowers grown in pots (substrate is soil) in later stages of the plant development. Can anyone tell me, what is the best...
29 April 2024 8,775 1 View
Hi, Does my TaqMan probe recognize the gene only if the sequence has 100% coverage with the probe or will I also get signal if there is a SNP or even a few bp difference? And if a few bp are ok...
09 April 2024 6,856 3 View
I have tested enzyme activity (b-galactosidase) with cation ions in different buffer and the results differ dramatically. For example, while I used MES buffer calcium acts as an activator but when...
11 February 2024 3,135 2 View
Hello, Does anyone have any kind of experience with ordering cell lines from Accegen? I'm wondering if they are trustworthy and about the quality of provided cell lines?
26 November 2023 3,757 1 View
I think that mentoring programmes support in meeting publishers' expectations.
05 October 2023 2,584 1 View
I need to get information about the number of chromosomes or DNA content. I have seedlings from in vitro that were frozen at -20 degrees after liquidation of the culture. They are seedlings from...
28 September 2023 6,373 2 View
I need to detect phosphorylated IGF1R in cell lysate and brain lysate with Western Blot. Are there any life hacks for detection of such proteins?
05 September 2023 7,310 0 View
https://webmagazine.unitn.it/en/evento/sociologia/115480/esa-rn12-environment-and-society-summer-school
10 March 2023 3,806 3 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...
06 August 2024 7,102 0 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
As described in the Multifractal detrended fluctuation analysis (MFDFA) algorithm, it at first calculates the profile of the time series, and then other steps are operated on the profile....
05 August 2024 9,366 2 View
I need the python code to forecast what crop production will be in the next decade considering climate and crop production variables as seen in the attached.csv file.
05 August 2024 2,977 3 View
I want to know how do you make real time pcr solation ? is there any machine to make it? thanks for answering
05 August 2024 1,660 0 View
Hello everyone, I am currently working on a research project that aims to integrate machine learning techniques into an open source SIEM tool to automate the creation of security use cases from...
04 August 2024 3,196 2 View