I start the analysis of gene expression and I do not know how to plan the experiment. I know about the dilutions, color compensation but I do not know what is the calibrator and what material I should use for that?
Thank you, yest it helps.
Dear colleagues, help me to understand the rules for authors. I am writing an article for journal "Climacteric". According to the rules for autors it is necessary to indicate "Subject...
09 February 2021 2,221 4 View
Hello everyone! I've encountered a problem when trying to figure out the methodology of my dissertation. The study has multiple variables and will be, in general, rather complicated, but my only...
02 February 2021 2,086 3 View
Hello, dear colleagues! I would ask you for a little help. Actually, I'm doing a multivariate linear regression analysis in the SPSS and one of the independent variables has negative values. In...
20 December 2020 535 2 View
To prepare a sample for ICP-Analysis one has to dissolve the material in the chosen liquid and then filter it. How can I consider the loss of mass after filtration if I don't know it exactly? I...
20 December 2020 4,394 3 View
I have done my analysis in R using the piecewiseSEM package and have got the results but I am unsure how to report it APA style. What values do I report? Thank you in advance!
13 December 2020 3,048 3 View
I'm interested in the results of Objective Yield Survey by USDA NASS in a raw form of plot-level observations. As far, as I understand, quickstats...
19 November 2020 5,369 3 View
I need to immobilize 3 peptides: CGPDGRDGRDGRDGR, CRQILIWFQNRRMKWKKYQRLC, penetratin peptide RQIKIWFQNRRMKWKKGG on the SiO2-covered gold nanoparticles. What is the most efficient and stable way...
16 November 2020 2,909 3 View
I would be very grateful for any help regarding my problem. My research requires applying betulin (and betulinic acid) solution on the cerebral cortex slices of rats. With the stock solution of...
27 September 2020 8,758 2 View
Hello, I have questions about XL30 SEM (with tungsten filament). Can anybody help me with how to correct crossover? You will see in the picture look of my crossover. I trying everything and can't...
24 May 2020 1,339 3 View
Hello everybody, I have a qestion about the implementation of the XRF to determine the thickness of the NiP -Cr coating on a stainless steel (1.4305). According to the TDS of the base Material,...
24 February 2020 5,574 2 View
Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...
03 March 2021 687 1 View
Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...
02 March 2021 4,029 3 View
Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...
02 March 2021 2,616 1 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...
01 March 2021 6,999 3 View
To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...
01 March 2021 8,683 4 View
Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?
01 March 2021 5,311 1 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...
28 February 2021 2,133 3 View
Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.
28 February 2021 5,008 4 View