I want to detect cholesterol by colorimetric method. What buffer should I use?
Okay thanks for your valuable answer sir. I want to know is there specific buffer system for specific analysis like for my case cholesterol colorimetric detection using nanozyme peroxidase activity.
I have my RNA samples eluted in nuclease-free water. But now I want to store it for a longer period. Can I add isopropanol directly to my samples to store it without doing a second round of TriZol...
23 June 2024 2,576 4 View
I want to know about water contact angle of silica films without using any surfactants.
29 May 2024 4,526 3 View
supercapacitor application
13 May 2024 5,612 1 View
I was not very familiar with material studio software. I have run the DOS calculation, but I can not find the Fermi energy in the DOS output file. I have to do the Fermi energy level correction...
20 April 2024 8,223 0 View
While conducting experiment following data were obtained Compressor inlet pressure : 120kPa Compressor inlet temperature: 29°C Compressor outlet temp:1000kPa Compressor outlet temperature: 66°C
07 April 2024 3,045 4 View
I am trying to purify this eukaryotic protein (mol wt 60 kDa) in Rosetta strain. It is getting expressed in good quantity. However, there is another stubborn host protein (mol wt around 70 kDa)...
01 April 2024 3,961 3 View
Hi. I had written a manuscript on Bibliometric Analysis related to oral cancer last year. Since then I had sent it to multiple journals only to get rejected. I request you all to please give me...
04 March 2024 2,924 5 View
I along with my friends have been doing experimental analysis on VCRs by adding heat before and after compressor for our fourth year project. We have been observing that COP of our system is...
20 February 2024 6,133 1 View
I want to analyze degradation intermediates of phenolic compound through GC-MS analysis which I had carried out in aqueous solution. For liq-liq extraction of phenolic compound from aqueous...
25 December 2023 1,205 4 View
I have synthesized cDNA using one microgram RNA template. Then, I diluted it to five times. Then, I did PCR using diluted cDNA with housekeeping gene act2. In PCR, I got nice bands in the gel....
27 September 2023 8,098 4 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...
07 August 2024 1,937 1 View
The stability of the Solar System is a complex subject that blends the classical framework of Newtonian mechanics with the modern insights provided by General Relativity (GR). Understanding this...
07 August 2024 2,569 1 View
A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.
07 August 2024 5,307 1 View
Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?
07 August 2024 4,616 0 View