What are the dominant factors contributing to the success of electric vehicles in the marketplace?

Early innovation by Toyota via the Prius established an early adopter crowd for electronic vehicles. Since, competition in many offerings: GM (Volt, Bolt), Nissan (Leaf), and the rise of Tesla set...

02 January 2021 1,787 13 View

Best purification method for recombinant protein in BL21?

Hi I need to purify a recombinant protein (IL6 21kDa). It's been produced in E.coli BL21 with a T7 expression vector, but it does not have a tag as yet. I was thinking of adding a his-tag and...

09 December 2020 2,200 8 View

Recombinant Interleukin 6 western blot, non specific bands?

Hi I've been trying to produce some recombinant human Interleukin 6. I sub-cloned the gene for human IL6 into a Promega T7 Flexi vector (native gene no tag yet) and confirmed sequencing and...

09 December 2020 2,340 4 View

What to do when an advisor is responsible for first authoring a publication as part of a research program, but the advisor never follows through?

This was another interesting case study I was reminded of after critically debating the rights and responsibilities of graduate students / faculty mentors, and recent responses to another case...

23 November 2020 1,718 3 View

Advice to Create a Stable Cell Line with a T-to-A Mutation?

Does anyone have a recommendation on a methodology to create a stable cell line with a T-to-A mutation in a protein coding gene? We should be able to use CRISPR to induce a double-stranded break...

07 October 2020 2,504 1 View

Is publishing 3 first author papers acceptable for 1 doctoral credit hour?

As part of completing doctoral studies I signed up for a single hour of 700-level credit, where the preparation of two first author submissions was the goal. 3 first three author papers were the...

04 October 2020 8,547 3 View

Resolving an ABD, what are your expereinces?

The dreaded ABD (all but doctorate). There are many reasons why researchers can get stuck in ABD status. To better understand how this situation arises and resolving it, this question is posed to...

02 October 2020 4,049 3 View

What are the new transformative means of communicating your research to a larger audience?

Research Gate, Citation trackers, and Twitter are all means to promote your research, outcomes, knowledge and future possibilities. But what are novel and transformative means to expand research...

01 October 2020 5,199 15 View

How can Artificial Intelligence improve energy efficiency and reduce emissions?

Efficiency and emission reductions are two identified related factors of energy - environment topics. How are you utilizing artificial intelligence (AI) towards more energy efficient systems...

21 September 2020 6,284 13 View

Given a TEM image and molar concentration of a given nanomaterial, what is the best way to convert to particle, surface area, and mass concentrations?

Often research reports will only include only one concentration metric when characterizing nanomaterials. However, often it is necessary to convert among different concentration metrics, does an...

28 August 2020 6,754 5 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View