I grew a Pseudoalteromonas sp. on Zobell ZM/1 medium enriched with the following carbon sources:
- one type with 3% Glucose
- one type with 3% Tween
They were subjected to '' cold-shock'' temperature settings. This involved exhibiting the plates to temperatures of 10oC and 25oC interchangeably every 24 hours, for 3 days.
These specific samples, when stained using the Anthony-direct-dry method, to visualize the EPS capsules, and viewed under 1000x magnification, contained some peculiar structures.
The plates were examined carefully, and did not show any sings of contamination. They seemed to only contain the isolate.
Interestingly, the cold shock treated samples, were the only ones that contained these peculiarities.
The other plates grown at stable temperatures of: 4oC, 10oC and 25oC did not show anything like this.
I was wondering if anyone else stumbled across something similar and could possibly enlighten me. It might not be that important, but I would really like to know what these are.
Thank you in advance.
I would guess that one of the components of the growth medium precipitated / crystalised due to the temperature shift. I don't think that it is "organismic" growth.
Cheers, Christian
I agree with Christian Q. Scheckhuber. The crystals formed are most likely derived from the culture medium (it seems to me that it may be glucose), and have nothing to do with Pseudoalteromonas.
I agree to the answers and of Scheckhuber. They might come from the medium or the staining solutions
Wirojne Kanoksilapatham
Firstly, I would like to thank everyone for their answers.
I agree that the unidentified structures are azoic. However, I am still unsure about what caused their formation.
Dear, Phil Geis: Yes, both media contained these structures, and both have been exposed to the cold-shock treatment. Other samples, kept at constant temperatures of 4, 10 and 25 degrees Celsius did not contain these.
Out of curiosity I took a look at the medium composition of Zobell/ZM1. There are many different salts in it. It is certainly possible that the crystals are formed by a particularly insoluble salt (e. g., calcium phosphate). Yeast extract could be the origin of the phosphate. But this is just an assumption.
Regards, Christian
I do agree with previous answers that they are purely crystals and inanimate matters
Hi,
It looks like crystal structure.
You may try the cold-shock treatment with a blank or uninnoculated plate of the same medium, to confirm if the objects originate from the medium alone.
By the way, how is the grow response of your organism under this cold shock treatment?
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