Staining germ cells obtained after dilaceration of tissue treated with vincristine showed the presence of 'filaments'.
Testicular tissues were exposed to the chemical drug 33 days before the dilaceration.
The staining was done with Harris Hematoxylin, ammoniated alcohol and Shoor.
• Do you think they are 'tubulin filaments' or spindle representation (vincristine being a spindle poison)?
• Did anyone ever notice this kind of phenomenon in testicular tissue or have you ever seen this kind of representation?
• Do you think it is possible to have such consequences more than a month after treatment?
Thank you in advance
Dear lodovic,
vincristine and vinblastine are so strong alkaloids have potentially effects on proliferate activety for different types of cells, I record these effects on peripheral blood lymphocytes and causes mitotic index reduction.
Interesting!
Theoretically they could be actin or tubulin filaments. Being detected after vincristine treatment could suggest tubulin, but I think you need to prove this through specific staining . Are you able to stain with Phalloidin or Tubulin Tracker or so?
Thank you very much Iman Hassan Ibrahim for your feedback.
It was indeed one of our ideas but we have very few slide of this condition. We will see if the phalloidine is expressed in these 'germ cells filaments' on fluorescence stainings.
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