How to lessen cell growth?

I am working on the 4T1 murine breast cancer cell line. from the first day I bought this cell line, the cell's growth rate is extremely high. I intend to make these cells resistant to a single...

24 January 2021 2,884 3 View

Are these fungi or is it just medium impurity?

I just thawed my 4T1 cancer cells and 24 hours after seeding the cells in the T25 flask there were somethings beside my cells. The culture medium is pretty clear and there is no more than 6 or 7...

28 December 2020 5,928 3 View

Can Darcy equation be considered as a form of Computational Fluid Dynamic (CFD)?

Hello Scholars, Hope all of you are well and safe, I know that the CFD is based on Novier-Stocks equations which consist of a time-dependent continuity equation for conservation of mass, three...

16 August 2020 3,291 6 View

Does anyone know how one can calculate the relative importance(%) or feature ranking of input parameters in the LSSVM (in MATLAB)?

I am using the Least square support vector machine (LSSVM) in MATLAB and want to know the relative importance or feature ranking of the input variables by percentage. Thank you!

05 July 2020 7,329 3 View

Organic or Chemical Fertilizers, Which emit higher nutrients (kg/ha) to surface waters?

Take a farmland with typical steep and cultivation (for example, rice), which is inundated for irrigation. Is there any difference between chemical or organic fertilizers on nutrient load...

29 March 2018 1,748 33 View

What % of intragenic SNPs regulate distant genes?

Does anyone know what percentage of single nucleotide polymorphisms within a gene body regulate expression of genes other than the one in which the SNP is found in? In a related question, what is...

24 January 2017 7,313 3 View

What is the reason for the changing of color from green to brown in preparation Graphene oxide by Hummers method?

I prepared Graphene by Hummers method but the color of my solution after adding KMnO4 and stirring for 2 hours doesn't turn to brown. I know changing color is an important stage

24 June 2013 1,521 10 View

Hello! What is te racial/ethnic composition of this study? Very curious to know if there is any chance to look at some of the cultural variations?

Greetings! Very interesting topic. I have studied cultural and racial differences in correlation between positive and negative emotions. Just wondering if your data have enough of various racial...

01 January 1970 5,278 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View