What should the village government do in dealing with the spread of the covid-19 virus?

I am currently researching village government policies regarding the spread of the covid-19 virus, I want to gather opinions from various villages or areas affected by the Covid-19 virus, what...

07 September 2020 303 5 View

How to get ICRG (Economic Risk ratin) data ?

Hi, I'm a management student that want some help for finding the excel data of Economic Risk Rating from 2014 to 2019 or at least to 2018. If there's anyone who can help me please share the data...

13 January 2020 1,099 1 View

Can anyone identify the genus or family of these species ?

Im currently working on gut content analysis of some juveniles from seagrass bed in Seribu Archipelago, Indonesia. I found some difficulties as most diets were found in not complete part and...

17 June 2019 769 11 View

Can anyone help me to identify the genus of these crustaceans?

these crustacean found in a gut content of Ostorinchus margaritophorus (Apogonidae)

25 March 2019 7,462 22 View

How to measure reliability and validity test for experimental reseach?

i need references about how to determine the proper reliability and validity test for experimental research. i do research about analyzing validity and reliability of smartwatch for healthcare. it...

08 November 2018 2,948 3 View

Why a primer can produce different size of PCR product?

I have a primer of Ubq 1 and I used it to amplify two different rice cDNA groups by PCR. The first group samples produce single band around 200 bp while second group sample produce single band...

20 October 2017 885 4 View

I have a problem about classification secondary metabolites from natural products, may I ask for advice on the latest and reliable references ?

I am writing a bachelor thesis, and I am in a state of confusion in writing a literature review, as many sources with different classifications

06 May 2017 7,294 2 View

Is it possible to analyze the shut in pressure that recorded at the well head?

i know there must be the wellbore volume effect along the pressure record at the surface. but is it possible to analyze the shut in pressure recorded at well head to obtain the reservoir...

22 March 2017 113 3 View

Tricine SDS PAGE smearing?

Hello everyone, I have condition in my sds page tricine here. My sample is recombinant human epidermal growth factor (protein 6,2kDa). before going to sds, all the sample have to be extracted...

06 February 2017 7,297 11 View

How poly IC in transfection?

29 May 2016 7,885 2 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View