Could someone explain What are the structural aspects of PNA-DNA/RNA interactions in detail?
I would like to begin SPPS. The purity of DMF could cause issues while coupling if there is any purity. Although we have purchased DMF for peptide Synthesis. However, I would like to check the...
05 June 2016 1,037 4 View
The supreme property of chemical matter is its potency to have given rise to the emergence of life. Yet life’s origin continues to be one of the big unanswered questions of natural science. How...
03 April 2016 4,493 2 View
I'm an Organic Chemist experienced in the synthesis, characterization and crystallographic study of organic peroxides looking for Professors with the fund and PDF position in their group. I have...
08 September 2015 10,064 0 View
How to make 2 moles of a 35% by weight hydrogen peroxide solution from 30% hydrogen peroxide solution? If the procedure asks for 2 mole of H2O2. Then can we calculate the weight of that much...
02 March 2015 2,392 8 View
We have crystallized some bioactive organic molecules. On the basis of van der Waals radius of oxygen we found them to possess remarkably short intramolecular oxygen oxygen interaction. Is IR and...
10 November 2014 8,150 1 View
If a compound dissolves by heating any compound till it's melting or to its boiling point in any solvent, can we say it's soluble? Is there some relation between the concept of saturation,...
09 October 2014 5,523 5 View
We have crystallized some bioactive organic molecules. On the basis of van der Waals radius of oxygen we found them to possess remarkably short intramolecular oxygen...oxygen interaction. Further,...
09 October 2014 8,636 7 View
While working on peptides, we protected the amino acids and peptides. Even the very basic Boc protected Valine or Tyrosine is a thick oil which we use in the next step without purification. Even...
07 August 2014 6,794 4 View
Could organic peroxides have some role in the origin of life? Can we have some critical and rigorous discussion whether in favor or against any role of peroxide in origin of life.
04 May 2014 5,583 2 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View