How to determine purity of DMF by UV for SPPS?

I would like to begin SPPS. The purity of DMF could cause issues while coupling if there is any purity. Although we have purchased DMF for peptide Synthesis. However, I would like to check the...

05 June 2016 938 4 View

What is the chemistry of the rTCA and its role in Origin of Life?

03 April 2016 4,360 2 View

Could someone suggest a research group or professor with the fund and PDF position in their group?

I'm an Organic Chemist experienced in the synthesis, characterization and crystallographic study of organic peroxides looking for Professors with the fund and PDF position in their group. I have...

08 September 2015 9,949 0 View

How to make 2 moles of a 35% by weight hydrogen peroxide solution from 30% hydrogen peroxide solution?

How to make 2 moles of a 35% by weight hydrogen peroxide solution from 30% hydrogen peroxide solution? If the procedure asks for 2 mole of H2O2. Then can we calculate the weight of that much...

02 March 2015 2,222 8 View

How can I obtain the electron density of any organic compound experimentally & determine the O-O coupling constants between two O atoms?

We have crystallized some bioactive organic molecules. On the basis of van der Waals radius of oxygen we found them to possess remarkably short intramolecular oxygen oxygen interaction. Is IR and...

10 November 2014 8,038 1 View

What is the criteria for determining solubility?

If a compound dissolves by heating any compound till it's melting or to its boiling point in any solvent, can we say it's soluble? Is there some relation between the concept of saturation,...

09 October 2014 5,423 5 View

Does anyone know a protocol to measure the intramolecular interactions energy between two oxygen atoms?

We have crystallized some bioactive organic molecules. On the basis of van der Waals radius of oxygen we found them to possess remarkably short intramolecular oxygen...oxygen interaction. Further,...

09 October 2014 8,507 7 View

How to get (or crystallize) solid amino acids derivatives and peptides?

07 August 2014 6,622 4 View

Could organic peroxides have some role in the origin of life?

Could organic peroxides have some role in the origin of life? Can we have some critical and rigorous discussion whether in favor or against any role of peroxide in origin of life.

04 May 2014 5,483 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

What is involved in morphological changes of SEM images of a substance?

In the sem analysis of CaCO3 nanaoparticles, the morphology images showed spherical shaped particles but the proposed morphology for these type of nanoparticles is trigonal. I prepared the...

01 March 2021 1,960 4 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Microstrip antenna design tutorial of HFSS 3D layout module ?

I have designed a 24 GHz rectangular antenna on HFSS 3D model and got desired results while desing the same antenna with same dimensions I am getting resonance frequency at 27 GHz I am...

28 February 2021 4,426 2 View