We are expressing B-amyloid peptids and after purification we are having some problems with the aggregation of the samples. I'm wondering if there are optimal conditions of storage that dont include liophilization of the sample.
Lyophilization of the peptide is the best way for storage. When lyophilization is not available, the purified fractions may be may flash-frozen and stored at -80 °C; however, the peptides should be run through a gel filtration column before you perform the assay.
Hallo Maria,
my colleagues had found out that the monomer of amyloid beta peptide is rather stable in hexafluoroisopropanol (HFIP) and oligomers/ aggregates can be centrifuged off.
Here is the article
Monomeric Amyloid Beta Peptide in Hexafluoroisopropanol Detected by Small Angle Neutron Scattering
PLoS One. 2016; 11(2): e0150267.
doi: 10.1371/journal.pone.0150267
Regards,
Andreas
I agree with Mahafuzur Rahman, but you have to make sure that your sample stays frozen during the prozess. Otherwise you promote the Aggregation. Storrage of monomers can be done in HFIP or DMSO at min. -80°C with flash freezing. First you should check which form of Aß you have after your isolation, Aß monomers aggregate rapidly in "normal conditions".
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