Maria Cecilia Morcillo Muñoz

4 Questions 2 Answers 0 Followers

Questions related from Maria Cecilia Morcillo Muñoz

How can I replace the ribose sugar with deoxyribose using the AutoPSF plugin on VMD?

I'm trying to replicate a protocol from "Three-dimensional modeling of single stranded DNA hairpins for aptamer-based biosensors" and I'm having some problems with the 3rd step which includes the...

07 April 2020 7,144 0 View

How can I stain E. coli pLysS without cell lysis when I use permeabilizing agents?

I was using Triton x-100 before, but when we start using the pLysS bacterias it was imposible to keep using it. What we are trying to do is to stain inclusion bodies with an amyloid protein but we...

07 April 2020 296 0 View

What are the storage conditions that prevent aggregation in B-amyloid peptids?

We are expressing B-amyloid peptids and after purification we are having some problems with the aggregation of the samples. I'm wondering if there are optimal conditions of storage that dont...

08 March 2020 9,486 3 View

How can I disaggregate proteins?

I am trying to do a kinetics characterisation of AB-40 and AB-42 (they are recombinant peptides produced in the laboratory) but it seems that the samples ar alredy aggregated when I try to do the...

25 February 2020 7,563 4 View