What are the steps for preparing a water extract from the leaves of some plants and diluting them to 1: 1, 1:10, and 1: 100?
hello,
Extraction by grinding fine powder from the leaves or the whole plant followed by sonication is a rapid technique compared to maceration (which lasts several hours or even days). The extract is then centrifuged, and the supernatant is recovered and then subjected to vacuum drying (preferably). The resulting solid extract will be taken up with the chosen volume of water.
Grinding the material to be extracted followed by ultrasonication or soxhletion with water at least 1-2 hrs, the extract obtained can be further diluted with water
I agree with the answer of Abderrahmane zekhnini and ofcourse the dry extract will be diluted
steps
1. Grinding fine powder from the leaves
2. Mixing with DW.
3. Extraction technique (Sonication or other technique)
4. Dilution with DW.
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