I have limited samples for my analysis and I can only perform my analysis once in triplicates (or at least twice in triplicates not more than that). So far, my analysis showed persistent results. Is performing once in triplicates enough to conclude that my active samples have antimicrobial activities? Or should I perform the same protocol twice in triplicates or triplicates in three independant experiments? Your golden opinion is highly appreciated.
Veeramuthu Duraipandiyan
If you have limited samples, better to go concentration level is for pure compound in below 50 microgram, crude metabolites mean maximum 5 mg. This you can do triplicate test.
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Peter M Muriana
It would help if you explain exactly what you are looking for and how you are plating.
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