How to clean the Bio-basic SEC 60 (model no- 73305-307846) column?

Hello everyone, We are using the Bio-basic SEC 60 column to further purification of protein fractions using HPLC analysis. Sample initially separated via membrane filtration and less than 1kDa...

08 July 2020 6,453 1 View

How to analyse Dielectric function graph obtained from VASP to find the effect on solar absorption ?

I have calculated the frequency dependent dielectric function of cubic CH3NH3PbI3 using VASP. Imaginary and real parts of the dielectric function have been graphed as below. I need to know the...

06 November 2019 6,417 3 View

How to find frequency dependent dielectric properties of Hybrid perovskite (Eg. CH3NH3BiI3 , CH3NH3SnI3) ?

This is my first attempt to calculate dielectric function. I was trying to calculate dielectric function using VASP as it is advised in the tutorial in VASP. Its a 3 step process. 1.Standard...

23 August 2019 4,582 4 View

How to convert XYZ file to Gtif file by using gdal.?

I have 533 XYZ files. i want to convert these all files in to gtif files. i am looking to convert these all files together. Do you have any java or python script to convert these all files together.

25 February 2019 8,928 6 View

Is it possible to sequence whole genome from naturally shed bird feathers?

Hello, I'm trying to develop a proposal to investigate the genome of a wild bird species. Since these birds are very fragile and active, very hard to catch. So I'm considering some non invasive...

29 November 2017 6,596 2 View

How many GCMs would you use if you are making a model ensemble for climate change impact assessment ?

15 February 2015 905 5 View

When deriving the precipitation data from NETCDF files of CMIP5 GCMs, should I interpolate or just get the nearest point ?

In the climate change studies, we have to use CMIP5 data sets in NETCDF files. Should we interpolate between two points when getting prcp data for a point OR get the nearest point ? What is the...

17 July 2014 8,744 6 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

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22 February 2021 9,932 11 View

Is it okay to mention you distributed your qualitrics study link through social media sites?

Just want to be clear and know it is okay to say I have distributed the qualitrics link through social media such as Facebook, Instagram and Twitter to ensure my target audience is reached in my...

21 February 2021 5,143 2 View

What is the minimum number of exosomes/ml to have a good yield of total RNA for Illumina sequencing?

I am using IZON qEV2 columns to isolate exosomes from 100 ml of cancer cell conditioned medium. in NTA analysis I usually see concentration of 2x10^9 particles/ml. But when I proceed with this...

20 February 2021 6,879 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

Any suggestions or recommendations on a good publishing house based on the African for academic books in the field of Communication and media studies?

I am currently working on a book on Public Relations (from an African perspective) and the rationale behind the book is the fact that as instructors in this field, we borrow instances and case...

19 February 2021 2,482 2 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

Are there papers on?

I'm looking for a papers concerning either Christianity or neopagan religions and social media. What role does social media play in the spread of these religious movements? Any suggestions on the...

15 February 2021 655 4 View

SPSS: A scale with multiple items (7-point Likert) across 4 test conditions. How can I correctly summarize it?

Hello, I am currently writing my master thesis where I have a 2x2 factorial design with one downstream variable (2 independent and 2 dependent variables). I need to conduct a multivariate...

11 February 2021 6,014 4 View

A film coverage that allows for transmittance of wavelengths on the infrared scale?

I've landed on needing some type of highly optically transparent film or cover material that prevents evaporation of culture media; I am not sure which subfield of materials science would be able...

08 February 2021 5,760 1 View