Rajamanthrilage Kasun Madusanka

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Questions related from Rajamanthrilage Kasun Madusanka

Has anybody experienced small, viable, and round shaped organisms in Leishmania cultures?

I have used 1% penicillin/streptomycin and 20% FBS to supplement the M199 media. Those organisms are slow-growing and later on affect the growth of the Leishmania parasites also. Usually they take...

17 November 2021 3,188 5 View

What are the solvents that can be used instead of Triethanolamine, to dissolve Hemin in M199 media preparation for Leishmania cultures?

We are curently having a problem with activating cryopreserved Leishmania cultures as they dont seem viable after the addition of M199+20%FBS+0.5%penstrep (even after 2 weeks). So we are trying to...

18 September 2021 814 3 View