Dear all,

I recently used Cluspro server to dock my proteins and pymol interface residues script for a prediction of amino acids involved in the complex. Now, I want to mutate few amino acids and want to see how it changes the binding energy score as compared to wild type.

Could you suggest few softwares that I can use to predict the effect of mutations? I tried to use BindProf but it did not let me add two mutations at a time.

Also, how do we select the best figures after docking analysis? is there any cut off model score that can be considered as a parameter?