What are the safety requirements when using nano-carbon tubes for reinforcing Polymeric matrix?

More Al-Badrawy A. Abo El-Nasr's questions See All

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

Can I use two IRIS in the same plasmid expression cassette ?

I am trying to express m6A protein and a fluorescence one RFP in the piggyback vector. I was thinking first of using the P2A to separate between the m6A and the RFP protein but then I was afraid...

12 March 2024 9,713 0 View

Do I have to purge my DMSO with an inert gas to make my solution?

I am trying to dissolve 25hydroxycholestrol and GW 3965 in DMSO for cell culture (they are LXRs agonist). The product form for 25hydroxycholestrol and GW3965 says "it is soluble in DMSO, which...

25 February 2024 5,925 0 View

What is cause of qPCR curves to be sharp?

Hi I am getting these weird curves on qPCR Anyone knows why ? I tried to decease the primer and tablet concentration No change.

18 December 2023 5,105 2 View

Seeking a General Protocol for Inducing Cell Lines with TNF-a?

I'm exploring the induction of cell lines using TNF-a and would greatly appreciate any guidance on a general protocol or established methods for this process. Has anyone successfully induced cell...

11 November 2023 1,753 0 View

What are the recommended protocol for inducing bronchial epithelial cell line with TNF-a?

Which stage of passage in bronchial epithelial cell lines is most suitable for inducing inflammation using recombinant TNF-a? I'm seeking guidance on the optimal cell growth phase for this...

09 November 2023 3,838 0 View

Tips for a uniform IrOx-coated Ti mesh electrode?

I am trying to prepare an IrOx-coated Ti mesh (0.002'' thickness) by the dip coating and thermal decomposition method. Literature indicates that a well-coated Ti mesh with IrOx should show a dark...

07 August 2023 4,313 0 View

Why cells are stressed and how to eliminate this stress, can cells become healthy again with passage?

I am working with veroe6 for a while and every time I receive a new healthy flask one or two passages and cells become stressed. Please any one will give help why this happens and how to prevent...

19 July 2023 2,394 1 View

Can anyone provide information on culturing a bronchial epithelial cell line?

Does it work with ordinary mediums, or it requires a specific medium? According to the ATCC website, they recommend using their product exclusively for culturing it.

11 July 2023 3,745 4 View

Do TaqMan® probes work with "Rotor-Gene Q" qPCR machine ?

Do TaqMan® probes work with a "Rotor-Gene Q" qPCR machine?

25 June 2023 9,545 1 View

Adhesion strength of coating?

How can I determine a good adhesion strength range for coatings on polymer surfaces, such as DLC on polymer substrates? Is there a specific threshold for adhesion strength (from T-peel tests)...

10 August 2024 942 3 View

How to define an anisotropic material with asymmetric elastic compliance/stiffness matrix in ANSYS APDL?

I need to model an anisotropic material in which the Poisson's ratio ν_12 ≠ ν_21 and so on. Therefore, the elastic compliance matrix wouldn't be a symmetric one. In ANSYS APDL, for TB,ANEL...

09 August 2024 5,048 2 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

Is it true that $\det(V(A))$ may be only $\pm 1$, depending on $n$, for the last symmetric tridiagonal matrix $A$?

One can try to generalize the Vandermonde determinant in the following direction: Let $A$ be any symmetric $n$-order square matrix. Consider its powers' diagonal elements $(A^k)_{ii}$ and...

08 August 2024 6,690 1 View

How to increase simulation box size?

We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...

08 August 2024 4,842 0 View

Is there an alternative to a multinomial regression which allows the DV to be non mutually exclusive?

I am trying to analyse data from a survey examining what variables affect teachers perceived barriers to incorporating technology into their classroom. I have 5 predictor variables however my DV...

06 August 2024 1,752 3 View

How to heat polymer for about 130° without oil bath ?

is there any other way to heat a polymer evenly other than oil bath if so what are those and how to use it

06 August 2024 947 1 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

Usage of internal standards in LC-MS/MS analysis?

Have you ever seen a LC-MS/MS method uses both internal standards and external standards (in matrix matching purpose) but the concentrations of internal standards are outside the calibration curve...

05 August 2024 3,084 6 View

Request a single Lecture notes for math as detailed as this that I can find in one place?

- The Existence/Uniqueness of Solutions to Higher Order Linear Differential Equations - Higher Order Homogenous Differential Equations - Wronskian Determinants of $n$ Functions - Wronskian...

03 August 2024 2,366 0 View