Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

How can we make other Machine Learning Algorithms Deep like Deep Neural Networks?

For example Decision Tree, Ant Colony Optimization etc.

26 December 2020 6,804 3 View

Please help me with this question?

In a heat exchanger, the cold stream is a corrosive material and it flows through the tube side. The shell side fluid is at a higher pressure than the tube side_ Recently one of the tubes in the...

01 December 2020 2,860 6 View

How adsorbed Mercury (II) react with HCl and HNO3 in water?

Case: Hg2+ is adsorbed on a titanium carbide and 0.1 M HCl/HNO3 is used for the desorption of Hg2+. During the reaction, the solution turns into a pale yellow color. I want to understand what...

16 November 2020 5,770 3 View

Help for data analysis?

I am working with raster data for ANN, i have converted rasters to numaric and then test train that numerical data and get very Good accuracy. now i need to convert my train data into raster using...

14 November 2020 9,902 2 View

How to link excel with Aspen plus?

Is there any way to connect excel data sheets with aspen plus as inputs to perform parametric analysis?? I want to change input parameters for a certain range to see system performance

28 October 2020 2,174 3 View

Help me about swelling behavior of graphene oxide nanocomposites with methyl methacrylate with different proportions of GO?

Swelling behaviour of PMMA with GO contents 0.1%, 0.2,0.3,0.5?

02 October 2020 2,655 3 View

Please share your ideas and research for future PhD candidates in Meteorology research direction?

Hello Respected Researchers, I'm Looking forward to the ressearch community to share ideas and research work for future PhD candidates in Meteorology Direction related to Hydro Meteorology, remote...

22 June 2020 3,475 16 View

Share your ideas for future PhD candidates in Environmenal Sciences research direction?

Hello Respected Researchers, I'm Looking forward to the ressearch community to share ideas and research work for future PhD candidates in Environmenal Research Direction related to remote sensing,...

21 June 2020 1,539 28 View

Why I am observing a voltage drop from 2.4 to O V while discharging Lithium Sulfur battery?

I made a Lithium sulfur cell, with Sulfur/Carbon cathode and Lithium metal anode using 100 uL of electrolyte (0.6 M LiNO3 and 0.4 M LiTFSI in DME:DOL). I am using a Biologic VSP 300 battery cycler...

27 May 2020 1,232 5 View

How to find natural frequency of a bridge from vehicle induced vibrations?

i have frequencies of bridge that are effected by mass of vehicle. the problem is when any vehicle pass the bridge it would add the its mass to the bridge mass ultimately mass of the bridge will...

22 February 2021 10,004 3 View

The Role of Town Planners in CAV Use?

Colleagues and I are about to begin a project investigating the role of town planners and local authorities in establishing the introduction of connected and autonomous vehicles (CAVs). What...

21 February 2021 2,247 3 View

How to Measure Consumer Willingness To Change from petroleum-fueled vehicle to electric vehicle?

I wanted to calculate the probability of one consumer shifting from using a petroleum-fueled vehicle to an electric vehicle. Since my background is in engineering I do not know much about how to...

15 February 2021 9,190 5 View

How to extract the yearly cash flow data (not time-series) from HomerPro in Excel? Also how to extract the monthly average electric production data?

I want to plot the monthly average electric production data and cash flow data in another software to get a more clear graph. For this, I need to extract the monthly or yearly data and not the...

11 February 2021 2,740 2 View

How to get actual or true SoC while predicting SoC for Batteries using Data Driven Approaches?

I have seen it in multiple research articles that authors use certain approaches to predict electric vehicle battery's state of charge(SoC) and then they compare it with battery's true SoC. So I...

11 February 2021 9,070 3 View

Any open-access dataset for battery systems from the field available?

Battery dataset is more and more critical for battery research. Although some dataset from the lab test is available online, rare information about the dataset from the battery systems in electric...

05 February 2021 8,372 1 View

Dear all, Iam currently working on Dc Microgrid model for electrical vehicle charging. Can any body give good IEEE research paper on this topic?

please give any suggestion?

04 February 2021 4,837 5 View

Distribution of modes fields ?

Hi, We find a specific distribution of electric fields in structure for different frequencies which can be seen using em solvers and known as modes. I would like to know how we can choose the...

04 February 2021 10,043 3 View

Is it possible to report hydrodynamic loads on different parts of an ocean vehicle while 6DOF motion analysis in Ansys Fluent is running?

It seems a 6dof UDF containing vehicle characteristics such as mass and moments of inertia can be used for specifying a boundary as reprentative of ocean vehicle. It is required to have...

03 February 2021 7,726 3 View

About COMSOL , HOW TO COUPLE EQUATION?

26 January 2021 4,690 3 View