i want to isolate extreme halophiles from the soil samples. i tried with 10%, 20%, 30% nacl solution along with nutrient agar. is this procedure correct or can i change alternative procedure or media?
@ Navya, we use Potato dextrose broth which adjusted to pH 7.0 using 1M potassium hydroxide (KOH) and the media with Na (750 mM) and without Na (0 mM) were prepared; we then divided 5 ml of the media into 15-ml centrifugation tubes. Na concentration was set referring to Hotchkiss (1923). Individual bacteria were inoculated to each tube and incubated at 25°C for two days. After incubation, the optimal density for bacterial growth was measured at 600 nm. The strains which grew to more than half the number of those growing in the control (0 mM Na) tubes were selected as Na-resistant bacteria. If you want to use nutrient agar, then better to take 4-8% of NaCl concentration for the isolation from the soil.
an organism, especially a microorganism, that grows in or can tolerate saline conditions.we use Potato dextrose broth whichadjusted to pH 7.0 using 1M potassiumhydroxide (KOH) and the media with Na (750mM)NaCl.
I will try this procedure, Thank you J. C. Tarafdar Angham jabbar Alwan
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