Hello dear all,
What are the probable reasons for the change of normal blood glucose level in Swiss albino mice ?
Thank you for your help.
Hi dear all, thank you in advance for the answer.
04 May 2018 9,888 13 View
Hello dear researchers, I need a simple detailed protocol to induce diabetes in mice with alloxan. Thank you in advance !
04 May 2018 9,647 5 View
Hello dear researchers, I'm asking about the concentrations that I must prepare to analyse Organic Extracts (of polyphenols) by HPLC, and Essential Oils by GC,GC/MS. I have ideas (that...
02 March 2018 3,670 9 View
Hi dear all, I will administrate plant extracts orally (per os) to rats. What are the precautions ?, which kind of syringe should I use for both mice and rats ?, what if the oesophagus is...
02 March 2018 2,673 7 View
Hello dear all, The APJTBM is recently transferred to Wolters Kluwer India Pvt Ltd (it was edited by Elsevier). Do you know why and what does this mean ? .. do they have the same quality ? is the...
02 March 2018 4,836 8 View
Hello ! Some of the most interesting questions come from the imaginations of children .. For example .. When a child asks you, why the sky is blue ? .. if you give him the right answer that the...
01 February 2018 6,929 1 View
Hello dear resesarchers , Look at the appearance of my evaporated phenolic extract using a rotavapor. Is it normal ? Thanks for anwsers.
01 February 2018 9,178 5 View
Finding ways to treat these ailments is hard for science and medicine today.
01 February 2018 7,360 4 View
Hi !. I love learning new languages and I want to learn German at home. Can anyone help me ? ... give me advice. Thank you all.
01 February 2018 2,853 0 View
Hello, I want to know if it is possible to analyse my methanolic extract by HPLC before its evaportaion using a rotavap. If no, what are the reasons ? Thank you for clearing up this matter.
01 February 2018 344 2 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View