How to induce diabetes with alloxan in mice ?

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What are the probable reasons for the change of normal blood glucose level in Swiss albino mice ?

Hello dear all, What are the probable reasons for the change of normal blood glucose level in Swiss albino mice ? Thank you for your help.

05 June 2018 6,438 0 View

How much time do mice need to adapt to new living conditions ?

Hi dear all, thank you in advance for the answer.

04 May 2018 9,763 13 View

What are the adequate concentrations of Essential Oils and Organic Extracts to be analysed ?

Hello dear researchers, I'm asking about the concentrations that I must prepare to analyse Organic Extracts (of polyphenols) by HPLC, and Essential Oils by GC,GC/MS. I have ideas (that...

02 March 2018 3,568 9 View

How to administrate plant extracts orally (per os) in a good way for rats ?

Hi dear all, I will administrate plant extracts orally (per os) to rats. What are the precautions ?, which kind of syringe should I use for both mice and rats ?, what if the oesophagus is...

02 March 2018 2,549 7 View

Any information about the Asian Pacific Journal of Tropical Biomedicine ?

Hello dear all, The APJTBM is recently transferred to Wolters Kluwer India Pvt Ltd (it was edited by Elsevier). Do you know why and what does this mean ? .. do they have the same quality ? is the...

02 March 2018 4,728 8 View

How to answer children's questions ?

Hello ! Some of the most interesting questions come from the imaginations of children .. For example .. When a child asks you, why the sky is blue ? .. if you give him the right answer that the...

01 February 2018 6,827 1 View

What are the cures for the world's biggest deseases, like cancer, heart disease and dementia ?

Finding ways to treat these ailments is hard for science and medicine today.

01 February 2018 7,268 4 View

This result is it normal ?

Hello dear resesarchers , Look at the appearance of my evaporated phenolic extract using a rotavapor. Is it normal ? Thanks for anwsers.

01 February 2018 9,084 5 View

Can anyone help me to learn German ?

Hi !. I love learning new languages and I want to learn German at home. Can anyone help me ? ... give me advice. Thank you all.

01 February 2018 2,749 0 View

What are the most important difficulties you encountered in the laboratory ?

Hello, While working in the laboratory, what are the most important problems you've encountered ? ... what solutions have you taken ? Thanks for the answers.

01 February 2018 7,195 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View