It is possible to scan with an profilometer a round material with 10 mm thickness and 63,5 mm diameter?

The material is a composite with short unoriented fibers based on PMMA, ABS as a matrix, and fiberglass as reinforcement.

28 October 2020 2,890 2 View

Does anyone have any suggestion for useful software tools for academic writers?

I want to know if there are useful software tools that help researchers improve their time management for writing a paper.

14 November 2017 5,278 2 View

There is a statistical tool for comparing multiple scales?

I have 4 items to a questionnaire in which the answers were the same type of scale: from 0 to 5, 0 for always false and 5 for always true. I want to study if an item score can determine certain...

18 September 2016 7,456 3 View

Do you know examples of first order sentences true in Q and C but false in R?

08 October 2015 8,433 45 View

Does anyone know a chemical supplier where to buy K2O (potassium oxide)?

Or maybe a good recipe how I can make it in the lab?

11 May 2015 9,449 4 View

Are there any yeast cell wall suppliers out there willing to send me a sample or to ship me the minimum order quantity?

I am having a hard time finding a supplier that is willing to communicate even. Do you know of any?

20 March 2014 3,358 5 View

Any article referring to this project?

Dear Sir, Do you have any article published on this project subject? Thank you for your attention!

01 January 1970 862 2 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View