We are trying to measure the migration of neuroblastoma Neuro2a cells using xCELLingence system with CIM-16 plates. The plates have two chambers: the upper contains cells, the lower contains chemoattractant (serum). The measurement is done in the lower chamber with impedance microelectrodes. The normalization of impedance in the lower chamber is done before the addition of cells to the upper chamber. As cells move from the upper chamber towards chemoattractant in the lower chamber, the impedance is supposed to increase, or stay ~0 (if cells do not migrate).
However, we faced the problem that in some replicates cell impedance gradually becomes more and more negative. I provide the example of 6 replicates of the same condition.
This effect doesn't depend on specific wells and all the electrodes are functional. This effect was never seen in other cell lines that we usually measure (e.g. HUVEC)
What are the possible reasons for cell impedance to become negative?
Dear Anna A. Shmakova
Check this previous discussion:
Article Application of xCELLigence RTCA Biosensor Technology for Rev...
Dear Dr. Shmakova,
I am sorry not to have a better answer, but could you try a different growth matrices?
Best Wishes
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