I am looking for a provider for GMP NGS sequencing, but all the costs and TAT are hidden behind a quote system which makes it hard to choose a provider. Does someone have experience with this?
I have a .pdb file with a protein structure prediction from Alphafold, and I want to highlight the existing Uniprot feature annotations (phosphorylation sites etc.) in the predicted structure....
12 October 2021 7,948 1 View
You may also see JVSTA 8, 1990 p. 3123 for the adjustable model's immediate use; & JVST B11 (1993) p1895 for the application of the adjustable model to prediction and verification of Ar etch...
03 September 2019 7,000 0 View
I don't see how insulating titanium oxide can conduct electricity.
01 November 2017 342 4 View
Hello, For my graduation thesis I'm studying the dynamic behavior of a timber-concrete composite beam. I want to make a FEM-model using Abaqus. With my model I have the problem that the beams...
30 August 2016 4,209 6 View
This is a question to fellow researchers living in countries where English is studied as an additional or foreign language (EFL). Theories proposed by Ehri and Rosental (2007) as well as Perfetti...
01 January 1970 3,921 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
Hi all. As a beginner in ChIP-seq experiments, I hope you understand that the following questions might be somewhat basic. I am planning to perform ChIP-seq or MeDIP-seq analysis to investigate...
28 July 2024 6,938 1 View
Gene sequencing related trouble shooting
25 July 2024 4,149 2 View
In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....
24 July 2024 8,076 3 View
Hello researchers, Sorry for my stupid question. I am learning the QIIME2 workflow for analyzing some 16s amplicon NGS fastq data. I found a very nice paper with data and code public available...
20 July 2024 5,405 2 View
Hello all, I extracted RNA from my samples and performed RIP-seq. After annotating the genomic regions using R, I obtained promoters, exons, introns, and UTRs. Given that my samples consist of...
18 July 2024 1,579 2 View
How is biomass energy essential in the power generation in a country.
08 July 2024 9,477 2 View
Hi everyone, I am working on a hybridization chain reaction (HCR) and trying to visualize it with gel electrophoresis. The visualization of the HCR works but for the single components of this...
07 July 2024 9,413 4 View