What are the phenotypic (non molecular) methods for the detection of hidden metallo beta lactamase producer in Acinetobacter spp?

More Saroj Chandra Lohani's questions See All

How to target each allele of gene of interest in tobacco plant using CRISPR Cas9 so that gene of interest is perfectly silenced?

Since Tobacco is tetraploid does it is necessary to design gRNA in such a way so that all allele of gene of interest is targeted and we will have perfectly silenced gene. If so how to do so.How to...

03 April 2017 5,140 1 View

Is Antibiotic Resistance Proof of Evolution?

Can we consider the drug resistance capacities that bacteria gain is an example of evolution that we can see right in front of our eye?

10 November 2016 2,790 5 View

What are the ATCC cultures (gram positive and gram negative) should be used in testing antimicrobial activity of herbal plant Extract?

I have 10 MDR isolates (five each of gram positive and gram negative bacteria) including metallo beta lactamase producer isolated from clinical samples and I want to test antimicrobial effect of...

06 July 2015 2,162 3 View

How to calculate chi square test for more than 2 by 2 consistency table in SPSS?

If we want to calculate chi square test for 3 by 2 or 4 by 2 or more than 2 by 2 consistency table, what is the procedure in SPSS? If some cell has expected value less than 5 then what will be the...

11 December 2013 980 9 View

What is the mechanism beyond Aztreonam (Monobactam) resistance in Metallo beta lactamase (MBL) producing Pseudomonas aeruginosa?

Literature suggests MBL producers are not able to hydrolyze Aztreonam (Monobactam) but during my thesis work I found most of the MBL producer Pseudomonas aeruginosa isolates are resistant to...

10 November 2013 7,826 4 View

What is the Tigecycline disk diffusion breakpoint criteria for Acinetobacter?

Tigecycline is often used for the treatment by Acinetobacter but its breakpoint for Acinetobacter is not prescribed and most of the researcher use criteria for Enterobacteriaceae i.e disk...

07 August 2013 9,911 9 View

What are the Zone Diameter breakpoints of Colistin (25 microgram) for Pseudomonas aeruginosa?

07 August 2013 3,980 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

I have a problem with kanamycin - why the kanamycin doesn't affect?

Hi everyone, I need to expression of GFP with pET28 plasmid in E. coli BL21(DE3) plysE. Transformed cells have kanamycin and chloramphenicol resistance. Normally, only transformed cells should...

22 February 2021 4,032 4 View

How does elongation affect the resistance of an electrode?

What are the testing rate for electrodes recommended by ASTM for cochlear implants. I am currently doing the 15mm/min elongatio testing.

22 February 2021 1,515 1 View

Rémy A Bonnin

Good morning,

The gold standard to detect hidden beta-lactamases remains the molecular tests.

If you want to perform phenotypic tests, you can try do an E-test Imipenem supplemented with EDTA. The inhibition of carbapenem resistance mediated by EDTA could suspect the presence of metallo-b-lactamase. However, some false negatives occur. You can also try biochemical tests, e.g. the hydrolysis of imipenem by spectrometry UV with or without EDTA.

Some techniques and their efficiency are summarized in this review:

https://www.researchgate.net/publication/237095470_Screening_and_deciphering_antibiotic_resistance_in_Acinetobacter_baumannii_a_state_of_the_art?ev=prf_pub

Regards

Rémy A. Bonnin

Article Screening and deciphering antibiotic resistance in Acinetoba...

Saroj Chandra Lohani

Firstly Thank you for the infromation

I have used Imipenem disc and EDTA combination for the study of Metallo beta lactamase producer and Ceftazidime(30 mcg) was used as screening antibiotics. One of the spps of acinetobacter is resistance to screening antibiotics CAZ and sensitive to imipenem and zone of inhibition of IMP+EDTA is greater than imipenem alone by 5mm, then can I concluded phenotypically that that spps harbor hidden MBl.

I have not gone through the biochemical test as you stated eg the hydolysis of imipenem by spcetrometry UV with or without EDTA I'll take account on that and will go for literature review over that.

I have gone through the link you provide but it was not free full access but even its Abstracted could be helpful to me Thank you for your consideration and help.

Initially I have tried to download form the source(Pubmed) from where I was asked for the subscription (Expert Review of Anti-infective Therapy) but the link you provided of research gate I was able to download full article.

Thank you very much for the link. It will be very helpful for me to write my thesis paper on Acinetobacter.

Thank you very your time and help

You're welcome.

I would like to say you that be careful of false positive using IPM+EDTA. Due to the intrinsic effect of EDTA on Acinetobacter, you could have some false positive result. You have to verify the presence of MBL by molecular test. In the review, you can find some resistance phenotype linked to the production of MBL.

I have gone through the review article and it was very helpful to me on my thesis work. I will take consideration on false positive due to intrinsic effect of EDTA on Acinetobacter.

Thank you for your help Dr Remy Bonnin sir.