Saroj Chandra Lohani

8 Questions 20 Answers 0 Followers

Questions related from Saroj Chandra Lohani

How to target each allele of gene of interest in tobacco plant using CRISPR Cas9 so that gene of interest is perfectly silenced?

Since Tobacco is tetraploid does it is necessary to design gRNA in such a way so that all allele of gene of interest is targeted and we will have perfectly silenced gene. If so how to do so.How to...

04 April 2017 5,264 1 View

Is Antibiotic Resistance Proof of Evolution?

Can we consider the drug resistance capacities that bacteria gain is an example of evolution that we can see right in front of our eye?

11 November 2016 2,877 5 View

What are the ATCC cultures (gram positive and gram negative) should be used in testing antimicrobial activity of herbal plant Extract?

I have 10 MDR isolates (five each of gram positive and gram negative bacteria) including metallo beta lactamase producer isolated from clinical samples and I want to test antimicrobial effect of...

07 July 2015 2,241 3 View

How to calculate chi square test for more than 2 by 2 consistency table in SPSS?

If we want to calculate chi square test for 3 by 2 or 4 by 2 or more than 2 by 2 consistency table, what is the procedure in SPSS? If some cell has expected value less than 5 then what will be the...

12 December 2013 1,159 9 View

What is the mechanism beyond Aztreonam (Monobactam) resistance in Metallo beta lactamase (MBL) producing Pseudomonas aeruginosa?

Literature suggests MBL producers are not able to hydrolyze Aztreonam (Monobactam) but during my thesis work I found most of the MBL producer Pseudomonas aeruginosa isolates are resistant to...

11 November 2013 7,920 4 View

What are the phenotypic (non molecular) methods for the detection of hidden metallo beta lactamase producer in Acinetobacter spp?

Hidden Metallo beta lactamase in Acinetobacter spp.

08 August 2013 6,102 5 View

What are the Zone Diameter breakpoints of Colistin (25 microgram) for Pseudomonas aeruginosa?

According to CLSI guideline Zone Diameter breakpoints of Colistin (10 microgram) for Pseudomonas aeruginosa are ≥ 11 mm for sensitive and ≤ 10 mm for resistance but if we use Colistin (25...

08 August 2013 4,071 3 View

What is the Tigecycline disk diffusion breakpoint criteria for Acinetobacter?

Tigecycline is often used for the treatment by Acinetobacter but its breakpoint for Acinetobacter is not prescribed and most of the researcher use criteria for Enterobacteriaceae i.e disk...

08 August 2013 9,992 9 View