What are the optimum conditions for expression in bacillus subtilis (WASD ) ?

10 October 2018 0 6K Report

I am looking for the optimum conditions for expression or even surface display in bacillus subtilis (WASD chist ) ; And the plasmid that I am using is phy300plk for my construct . The problem is after too many tries I couldn't get any result (protein ). I'd be appreciated in advance if anyone gives me more information about that .

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