How can I be sure that my bone marrow cells are differentiated into bone marrow derived macrophages (day 7) at a gene expression level?

Hi everyone, I'm trying to establish a standard protocol to cultivate BMDMs. I'm culturing BM cells for 7 days in 100mm petri dishes, addition of M-CSF(20ng/mL) on day 0 and day 3. I would like...

09 July 2020 1,742 3 View

Insects and COVID-19: a possible contaminant?

Dear colegues, What do you think about this? Can insects or others arthropods transmit the virus (directly or indirectly)?

19 April 2020 7,391 53 View

How can I identify and catalog the Rainforest fungus?

We have a big area of Rainforest where we can see a big variety of fungos growing around. I'd like to catalog them using a simple methodology. We do not have equipment for that despite a...

16 March 2020 9,034 3 View

I tried metawin unfortunately I couldn't get much help on how to code studies. Does anyone know a good free package for correlation meta analysis?

By the way I also need advice on which correlations to extract from factor studies ( exploratory, confirmatory, & SEM PLS) for meta analysis?

16 March 2020 7,217 3 View

Deep Euthetic Solvents, how to prepare?

Hi, everyone. I am starting to work with fractionation of Biomassa by Deep Euthetic Solvents . I came across a DES that is comprised of Lactic acid: Glucose:water (molar ratio 5:1:3), as in ref....

12 March 2020 3,474 3 View

Forests Pests: increased damage by climate change?

Dear researchers, what is your opinion about climate changes and forests pests? there is an increase or reduction of damage caused by insects and mites on trees?

29 February 2020 2,434 17 View

How to deal numerically with the discontinuities coming from a Butler-Volmer interface condition in a secondarry current distribution model??

Let us suppose that we want to compute the secondary current distribution of an electrolytic cell composed by anode, electrolyte and cathode. The governing equation in each subdomain isgiven by...

17 February 2020 7,913 3 View

No title

11 February 2020 3,076 10 View

How do I read the output files for a TDDFT (UV-Vis) job calculation (specially the graph itself)?

Hi everyone, so, I did some TDDFT calculations and have my .log files. I know I should be looking for the oscillator strength, the energies in eV and so on..., but my problem is this: How can I...

05 February 2020 7,783 3 View

Can one conduct a meta analysis on previous meta analyses?

When meta analyses on the same topic yield different results with no consensus, can one conduct a meta analysis on the Meta analytic studies?

19 December 2019 6,113 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

What is the best method to evaluate and measure non-circulating estrogen levels in mice ?

Could anyone suggest me the best procedure to measure the circulating estrogen levels in mice ? I came across the general methods where it can be measured in serum or urine but a detail suggestion...

22 February 2021 4,352 2 View

Questio regarding micro/nano particles fabrication ?

I will start to fabricate micro/nanoparticles for growth factor release and I check out a procedure quite nice, however, it is lacking some details and since I don't have much knowledge doing this...

20 February 2021 199 2 View

Hi..how to isolate crude HDL (all subfractions) from human serum by size exclusion chromatography?

I would like to know about isolation of crude HDL from serum by SEC. Is it possible to perform with a serum sample volume of

19 February 2021 5,576 2 View

How to successfully revive cells stored at -80 degree C for a year?

I am working on C6 cells along with other neuronal cell lines. We have preserved the cells in 90%FBS and 10% DMSO solution. We are reviving the cells after a year and cells are either not binding...

18 February 2021 4,499 1 View