What is the possible treatment for Motile Aeromonas Septicemia in fish?

According to Mahon & Lehman (2019), Aeromonas are distributed in a wide range of marine and freshwater environments worldwide. One way of getting infected by Aeromonas is through the ingestion...

18 October 2020 5,564 2 View

Which ordination method and distance/dissimilarity measure to use for proportionate plant cover data?

Sooo I have a data matrix with my sites in the columns and the species across the rows. I have about 200 different species. For each species I measured its proportion (out of 100%) in each of the...

06 May 2020 4,092 3 View

To what extent do you need a detailed and / or a priori literature review for an inductive qualitative study?

I am new to transparently inductive research, coming from a much more quantitative background. Really trying to do it as thoroughly as possible as I can see it's often done badly! But there's a...

05 December 2018 2,973 9 View

What statistics should I used for Likert Scale?

Was wondering if anyone could help me, I am in my third year and I'm in dissertation hell. I have used 5 point Likert Scales in my questionnaire (Strongly agree, agree, so on). I'm now looking...

19 January 2018 6,122 5 View

Anyone know a hydrophobic UV-active compound?

I am creating a standard test solution to test the UV detector on my HPLC. Does anyone know a good (and hopefully cheap) UV active hydrophobic compound? EDIT: My apologies, I should have been...

11 December 2017 3,261 3 View

Common analytical challenges in industrial biotechnology?

14 September 2017 7,429 3 View

Does anyone have any experience using Click-iT EdU imaging kits for lymphocyte proliferation assays, if so what cell culture media do you recommend?

What cell densities do you recommend.

07 February 2017 2,968 3 View

I am looking into preservice early childhood educators and their preparedness to teach a full inclusion early childhood classroom, any information?

Also, wondering about the effectiveness of full inclusion for children age 3-4 identified with special needs. Currently exploring these two areas of interest to hone my dissertation topic.

02 February 2016 621 12 View

Help with Amyloid beta detection protocol (western-blotting)?

Hi everyone, I am a first year PhD student working on amyloidosis. I would like some tips and tricks to crack efficiently my current nightmare, i.e. the western blotting of the Amyloid beta and...

11 February 2021 2,513 3 View

Endothelial Functional Assays & Conditions?

Hi everyone, I would greatly appreciate it if someone can shed light on certain endothelial functional assays and their conditions. I often notice that protocols are run with serum-free media, or...

05 February 2021 7,276 1 View

What is the main difference between "CD105+ Endothelial Cells" and mesenchymal stem cells?

since CD105+ Endothelial Cells are CD31 negative, how do we differentiate them in cell culture from mesenchymal cells? is it using vwf? thanks a lot

03 February 2021 5,362 2 View

No signal/bands observed on western blot for ERK signaling?

My question is regarding a western blot experiment that I am performing to observe pERK signaling by stimulation with VEGF ligand (VEGF165 and VEGF121) on MDAMB231 cells and SW620 cells (both...

30 January 2021 1,039 3 View

What is the difference between human umbilical vein endothelial cells (HUVECs) and bone marrow endothelial cells?

Hello I am new to work on endothelial cells, and I have been reading about them a lot and trying to know the difference between human umbilical vein endothelial cells (HUVECs) and bone marrow...

28 January 2021 8,765 4 View

Is there a kit to detect COVID-19 in freshly isolated human primary cells?

Our lab harvests HUVEC (human umbilical primary endothelial cells) and endothelial cells can support COVID19 growth. The mothers will be screened for Covid, but we all know that there are false...

14 January 2021 8,530 2 View

How often should Mile Sven 1 (MS1) cells be passaged and at what cell density?

I'm looking for a culture protocol for MS1 cells (Mile Sven 1, ATCC: CRL-2279 ). This is an adherent endothelial cell line isolated from mouse pancreas. ATCC has a protocol, but it's lacking...

13 January 2021 4,175 3 View

How to remove the bubbles in Collagen gel and maintain the transparency?

I am working on the capillary formation of Lymphatic endothelial cells in a collagen matrix. But I am facing a technical problem to handle Collagen. I have made many times; I am not able to remove...

27 December 2020 5,495 3 View

Immortalized pulmonary microvascular endothelial cell to buy?

Hello, I would like to purchase an immortalized cell line for pulmonary microvascular endothelial cells (MVECs), but I could not find any. I looked up websites, like ATCC, Lonza, Cedarlane, and...

15 November 2020 8,085 3 View

Cell line that does not express any of the VEGF receptors?

I am looking for VEGFR-negative cell lines, that either show very low or no expression at all of VEGF receptors for cell signalling assays.

01 November 2020 6,046 1 View