What are the new methods of staining??

More Hadeel Basim's questions See All

How do I dilute 100% DMSO to a concentration of 0.1% to test a plant extract on cancer cells? What substance can I use for dilution?

please

20 March 2024 5,437 7 View

Is there a scientific conference that is not Arabic?

Hello I am looking for a scientific conference (non-Arabic) related to management, economics, tourism, or similar fields to publish research in for free or for nominal sums. As soon as possible,...

13 March 2024 8,664 0 View

Which is better to use in the evapotranspiration calculation ERA-5 or ERA5-land ?

Hello everyone, I would appreciate you helping me with this question, I am using remote sensing-based models to calculate ET actual. I want to use ERA-5 dataset as a meteorological dataset I...

13 January 2024 9,334 3 View

Any one help my friend about mathlab program to solve rate equation for quantum dot semiconductor laser ?

any one help my friend about mathlab program to solve rate equation for quantum dot semiconductor laser?

24 December 2023 2,831 0 View

Is sustainable tourism development a process whose purpose is marketing?

23 November 2023 8,526 5 View

Why the aspirin is not save for children!!?

It cause ulcer piptic and bronchospasm in general.

08 November 2023 2,575 0 View

How to compare two groups each group has before and after?

I have two groups each group has before and after readings (continuous data). I compared between the means using paired t-test in each group separately. and then compute the difference in each...

14 April 2023 8,371 4 View

Why The output of PySEBAL is not reasonable?

Good Morning/Afternoon I was running PySEBAL model and I examined the output of the actual ET, I found too many (no-data) pixels which I know from the sat image at the same date (see the...

30 March 2023 6,095 0 View

Does the window width of FFT filters affects the signals amplitude and their significancy ?

I noticed that by using small window width more significant peaks appear. How can I choose the width of the window minimizing the errors of taking a false significance?

27 March 2023 1,005 2 View

How Can I use the ESPA Bulk downloader python tool?

I placed an order using the ESPA interface to process about 20 satellite scenes, and I wanted to use the ESPA bulk downloader tool which is mentioned in the provided link espa / bulk-downloader ·...

21 March 2023 309 2 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

Senescence-associated beta galactosidase staining is False Positive in control group?

Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...

02 August 2024 6,735 1 View

Suggestions for the Annexin V Positive Control ?

Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...

29 July 2024 8,624 1 View

How can I get clearer background for my western blot?

I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...

29 July 2024 6,484 5 View

How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ?

Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...

25 July 2024 7,520 0 View

Ideal tissue thickness for multiple immunolabelling and confocal microscopy?

Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...

23 July 2024 2,844 1 View

What are those black/hollow structures?

Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...

21 July 2024 1,044 1 View

Fixation Problem with cancer cells?

Hello everyone, I work with polyacrylamide hydrogels, which I fictionalise with sulfo-sanpa and collagen I to then plate lung cancer cells. The problem is that I lose a significant amount of...

19 July 2024 3,906 0 View