What are the benefits exactly of synthetic intron (IVS) and internal ribosome entry site (IRES) on a plasmid?

Hello everybody! so I have a plasmid which has a synthetic intron (IVS) & an internal ribosome entry site (IRES), and I'm thinking to remove them as I'm cloning due to some matters related to...

26 January 2021 7,645 2 View

How we can calculated LOAEL and NOAEL for toxic pathological dose ?

how we can calculated LOAEL and NOAEL for toxic pathological dose

02 January 2021 1,175 2 View

Is there any handbook/video tutorial for learning Immunohistochemistry (IHC) slide analysis/ scoring ?

I want to know how to look/methods of(analyze and scoring) in different parts pathological section like stroma, Ductal epithelial, epithelial lining, luminal epithelial etc. in a different...

11 August 2020 984 4 View

I am working on COVID19 dataset??

I am working on COVID19 dataset, but, When I tried to convert the data.xlsx file to CSV file, I got an error. Also, i got an error when I upload the CSV file to WEKA. I tried to solve the problem...

05 May 2020 4,879 2 View

How can i define the USRP N210 on pc under windows 10?

How can i define the USRP N210 on pc under windows 10 and i used gnu radio 3.8.. i set the configuration and i make the ping and recived reply successfuly but i cannot define the device and an...

21 January 2020 1,442 2 View

What is the number of measurement required in Circulant and Toeplitz sensing matrix in general in term of N an M in compressive sensing?

what is the number of measurement M (the number of measurement vector that result from multiplication of sparse vector N with sensing matrix (NxM)) required when using Circulant and Toeplitz...

16 January 2020 9,666 2 View

Does Tobramycin have an amorphous structure?

I've been analyzing the crystallinity of Tobramycin by the XRD, it is supposed to have a crystalline structure, but when analyzed, it showed an amorphous structure

25 November 2019 5,145 1 View

I've been trying to test the antimicrobial activity of zinc oxide nanoparticles against P.aeruginosa. They exhibited no activity. Any one knows why?

the nanoparticles were purchased from sigma and they have a size range (35-50 nm). i tried to dissolve it in DMSO ( 5% of the nanoparticle solution) and i also tried to sonicate it for 30...

24 August 2019 9,678 10 View

Combustion Engineering second edition by Kenneth W. Ragland, Kenneth M. Bryden?

I looked for this book literally everywhere but I can't find it, dose anyone have a PDF copy of this book because I really need it...

01 April 2019 6,305 3 View

Is there any specific technique for mice brain fixative and histopathology slide process?

brain tissue slide process

14 January 2019 4,828 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View