What are the names of the genes encoding ubiquitin protein in algae?

Bahram Barati @Bahram_Barati

06 June 2017 0 4K Report

I am confused with the gene naming, will appreciate your advice.

Badges
Science topic

More Bahram Barati's questions See All

How stress causes the Fv/Fm to decline?

Why Fv/Fm value decreases when a photosynthetic cell exposed to stress? I need more molecular explanation

08 September 2018 4,357 1 View

What are the benefits of studying the response of similar taxa of organisms like algae from different geographical regions?

Can it help to understand adaptive strategies of them? Any idea? Thanks for your answers

02 March 2017 5,056 5 View

What DNA to use for making qPCR standard curve?

I am going to run qPCR for several target genes so that for absolute and relative quantification I need to make a standard curve. Do I need to use my target genes PCR product dilutions in order to...

02 March 2016 6,104 9 View

Can experts in Chlorella morphology help with the attached SEM image?

I have attached the SEM image of Chlorella, the highlighted cell only observed in the heat-treated cultures. I will appreciate if you share your information about that. Thanks.

01 January 1970 2,494 2 View

I have calculated ratio of Chlorophyll_a to Carotenoid before and after treatment. Should I consider error propagation??

independent t-Test is used to determine significant changes between treatment and control(each group 3 replicates).

01 January 1970 8,364 9 View

Is there any cases algae not using the nutrient from the wastewater and grow normally?

I am working on microalgae cultivation using waste water. The initial concentration of nutrients were less but the microalgae has achieved biomass growth of 2 g/L. The final concentration of...

08 August 2024 4,812 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Hello researchers, how can one get the optical density of a filamentous algae?

i am comparing the growth performances of filamentous and non filamentous forms. in a case where I can not count the cells of the filamentous algae using a hemocytometer, is it okay to do a...

30 July 2024 5,985 2 View

Is it common for heterotrophic bacteria to grow on Pro99 and SNAX media?

I am attempting to isolate picocyanobacteria from seawater (pre-filtered with a GF/D membrane) using the pour plate technique and filter plating method (Kearney et al., 2022) on Pro99 and SNAX...

30 July 2024 1,040 1 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View