How to separate large amounts of two Riboflavin-based compounds with similar solubilities?

I'm running a reaction in where riboflavin is derivatized with a crosslinker in DMSO. The final reaction mixture contains riboflavin (non reacted) and riboflavin-crosslinker compound (slightly...

13 December 2020 4,865 3 View

Are there studies (dyes or confocal laser scanning microscopy) on depth of penetration of citric acid into dentinal tubules?

are there studies (dyes or confocal laser scanning microscopy) on depth of penetration of citric acid into dentinal tubules?

12 October 2020 8,924 1 View

Simulation Parameters for p-type and n-type layers of a typical silicon soalr cell?

Hi, I would like to simulate a typical polycristalline silicon cell with p-type and n-type layers using a drift diffusion model. I have found a lot of references for intrinsic silicon parameter...

03 February 2020 3,203 6 View

Intrinsic tryptophan fluorescence of protein measured FI using a fluorescence microplater reader based on filter or monochromator?

I was wondering if microplate reader recording filter based flourescence emission spectrum are good to measure protein protein Intrinsic tryptophan fluorescence? How does it compare with...

13 January 2020 6,354 2 View

Can I use a monthly average solar spectrum to represent the spectrum of the month?

I would like to calculate the monthly average of the representative solar spectrum for each month based on minute spectral irradiance measurements. Can I consider this hypothesis? Has anyone made...

02 October 2019 7,380 4 View

Method for Identification of Bacterial Natural products by HPLC and LC-MS-MS using databases?

I would be grateful if you could please indicate public libraries used for the identification of natural products produced by bacterial using LC/MS/MS data and/or HPLC DAD analysis

16 September 2019 7,077 4 View

When you recommend to cut the heart in a murine models of heart failure?

Good evening everybody, I am working on a model of acute heart failure and I am planning to analyse heat shock proteins (western blot) in the right and the left ventricle. The mouse heart is very...

03 June 2019 7,528 5 View

Do you know successful Agriculture and Rural Credit Guarantee schemes?

Agriculture and Rural Credit Guarantee Schemes face additional challenges if compared with urban SME's Guarantee Schemes, especially related to climate, pricing and insurance issues. I would be...

17 March 2019 2,863 4 View

Can I substitute Qiagen Buffer QG (from dna extraction kit) for Buffer ERC (Minelute reaction cleanup)?

I want to perform clean up on my digests but currently out of ERC from minElute clean up kit. Can I substitute it with buffer QG ? These are all from qiagen. Somebody told me I could do this but...

04 October 2017 8,150 3 View

Thoughts on performing PCR on purified, digested dna that has already been amplified (PCR) prior to digestion?

02 October 2017 3,916 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View