Various constructs have been added to the transgenic lines (events) through gene gun as well as by coker cotton and events need to be confirmed for the particular gene as well as its stability of expression.
If you have a large number of lines, I'd suggest starting with RT-PCR (starting from DNase treated RNA as your cDNA template) for the specific insert you used to make the transgenic lines. After narrowing down the candidate lines, I'd confirm with RT-qPCR and/or a Northern blot to confirm transcription of your transgenic gene (or if you are producing a protein product for resistance then I'd use ELISA or Dot blotting). From there you should have narrowed down your candidate lines enough that you can manage virus assays without being overwhelmed. I am attaching a paper by Bai et al., where virus-resistant transgenic lines are screened for Potato virus X and Y resistance in potato.
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